The influence of maternal HIV and Mycobacterium tuberculosis infection on infant immune responses to childhood vaccinations
Background: Altered immune responses might contribute to the high morbidity and mortality observed amongst uninfected infants exposed to human immunodeficiency virus-1 (HIV) in utero. This study examined the influence of maternal HIV and Mycobacterium tuberculosis (Mtb) infection on infant immune re...
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ndltd-bl.uk-oai-ethos.bl.uk-6168052017-06-27T03:23:31ZThe influence of maternal HIV and Mycobacterium tuberculosis infection on infant immune responses to childhood vaccinationsJones, Christine ElizabethKampmann, Beate ; Wilkinson, Robert2013Background: Altered immune responses might contribute to the high morbidity and mortality observed amongst uninfected infants exposed to human immunodeficiency virus-1 (HIV) in utero. This study examined the influence of maternal HIV and Mycobacterium tuberculosis (Mtb) infection on infant immune responses to immunisation. Methods: 109 mother-infant pairs were enrolled from Khayelitsha, Cape Town, South Africa, and were followed for four months. Peripheral blood samples were collected from mother-infant pairs at delivery and from infants at 16 weeks of age, following routine immunisations. Responses to BCG antigens were measured using multi-parameter flow cytometry and multiplex enzyme-linked immunosorbent assays (ELISAs). Specific antibody levels to Haemophilus influenzae type B (Hib), pneumococcus, Bordetella pertussis, tetanus toxoid and hepatitis B surface antigen were determined by ELISA. Results: At birth, HIV-exposed, uninfected infants had increased frequencies of proliferating T cells expressing TNF-α and increased levels of TNF-α protein in cell culture supernatants; levels were highest amongst HIV-exposed infants born to Mtb sensitised mothers. IFN-γ levels were lower amongst HIV-exposed, uninfected infants compared to unexposed infants. Maternal Mtb sensitisation was associated with increased infant IFN-γ levels at birth; infants born to HIV-infected, Mtb-sensitised mothers had similar levels IFN-γ compared to unexposed infants. Following BCG vaccination at 6 weeks of age, the immune response to infant BCG vaccination was unaffected by maternal HIV infection of Mtb sensitisation. Amongst mothers, Mtb sensitisation significantly influenced the response to BCG-antigens in HIV-infected but not in HIV-uninfected mothers. HIV-exposed, uninfected infants had lower specific antibody responses compared with unexposed infants at birth, but had robust responses following immunisation. Deficits in humoral protection against vaccine-preventable diseases were observed amongst HIV infected and HIV-uninfected women. Conclusions: Antenatal HIV exposure was associated with alterations in immune response to vaccine antigens at birth, however HIV-exposed infants had comparable potentical to respond to immunisation.610Imperial College Londonhttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.616805http://hdl.handle.net/10044/1/14556Electronic Thesis or Dissertation |
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610 Jones, Christine Elizabeth The influence of maternal HIV and Mycobacterium tuberculosis infection on infant immune responses to childhood vaccinations |
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Background: Altered immune responses might contribute to the high morbidity and mortality observed amongst uninfected infants exposed to human immunodeficiency virus-1 (HIV) in utero. This study examined the influence of maternal HIV and Mycobacterium tuberculosis (Mtb) infection on infant immune responses to immunisation. Methods: 109 mother-infant pairs were enrolled from Khayelitsha, Cape Town, South Africa, and were followed for four months. Peripheral blood samples were collected from mother-infant pairs at delivery and from infants at 16 weeks of age, following routine immunisations. Responses to BCG antigens were measured using multi-parameter flow cytometry and multiplex enzyme-linked immunosorbent assays (ELISAs). Specific antibody levels to Haemophilus influenzae type B (Hib), pneumococcus, Bordetella pertussis, tetanus toxoid and hepatitis B surface antigen were determined by ELISA. Results: At birth, HIV-exposed, uninfected infants had increased frequencies of proliferating T cells expressing TNF-α and increased levels of TNF-α protein in cell culture supernatants; levels were highest amongst HIV-exposed infants born to Mtb sensitised mothers. IFN-γ levels were lower amongst HIV-exposed, uninfected infants compared to unexposed infants. Maternal Mtb sensitisation was associated with increased infant IFN-γ levels at birth; infants born to HIV-infected, Mtb-sensitised mothers had similar levels IFN-γ compared to unexposed infants. Following BCG vaccination at 6 weeks of age, the immune response to infant BCG vaccination was unaffected by maternal HIV infection of Mtb sensitisation. Amongst mothers, Mtb sensitisation significantly influenced the response to BCG-antigens in HIV-infected but not in HIV-uninfected mothers. HIV-exposed, uninfected infants had lower specific antibody responses compared with unexposed infants at birth, but had robust responses following immunisation. Deficits in humoral protection against vaccine-preventable diseases were observed amongst HIV infected and HIV-uninfected women. Conclusions: Antenatal HIV exposure was associated with alterations in immune response to vaccine antigens at birth, however HIV-exposed infants had comparable potentical to respond to immunisation. |
author2 |
Kampmann, Beate ; Wilkinson, Robert |
author_facet |
Kampmann, Beate ; Wilkinson, Robert Jones, Christine Elizabeth |
author |
Jones, Christine Elizabeth |
author_sort |
Jones, Christine Elizabeth |
title |
The influence of maternal HIV and Mycobacterium tuberculosis infection on infant immune responses to childhood vaccinations |
title_short |
The influence of maternal HIV and Mycobacterium tuberculosis infection on infant immune responses to childhood vaccinations |
title_full |
The influence of maternal HIV and Mycobacterium tuberculosis infection on infant immune responses to childhood vaccinations |
title_fullStr |
The influence of maternal HIV and Mycobacterium tuberculosis infection on infant immune responses to childhood vaccinations |
title_full_unstemmed |
The influence of maternal HIV and Mycobacterium tuberculosis infection on infant immune responses to childhood vaccinations |
title_sort |
influence of maternal hiv and mycobacterium tuberculosis infection on infant immune responses to childhood vaccinations |
publisher |
Imperial College London |
publishDate |
2013 |
url |
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.616805 |
work_keys_str_mv |
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