Studies of nucleobases, nucleosides and nucleotides by LC, CZE, LC-MS, CZE-MS and MS techniques

• Main Author: Gong, H.
• Published: Swansea University 2000
• Subjects: 572.072
• Online Access: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637073

This work employs RP-HPLC and CE separation techniques, soft ionisation MS techniques (ESI and APCI), and on-line LC/MS, CE/MS and MS/MS techniques to analyse both the standards and nucleic acid constituents in mice livers. Emphasis is given to the method developments and the application of these techniques to tackle and solve biological and chemical problem. Chapter one gives an introduction to mass spectrometry, liquid chromatography and its separation modes, and also introduces and discusses several LC/MS interfaces based on their advantages, restrictions and their applications. Chapter two introduces capillary electrophoresis, its separation modes and CE/MS interfacing techniques with discussion of both sheath-liquid based on sheathless based CE/ME interfaces. Chapter three describes the analysis of nucleobases, nucleosides and nucleotide standards and nucleic acid constituents of the mice livers by on-line LC-APCI/MS and tandem MS/MS techniques, LSQ ion-trap and TSQ 700 were used to carry out the work above. Chapter four investigates fully and characterizes nucleobases, nucleosides, nucleotides and their isomers in several solvent systems by electrospray mass spectrometry (ES/MS). Develops analytical methods with LC-ES/MS, LC/MS/MS techniques and applies these techniques to examine the standards and identify several nucleobases, nucleosides and nucleotides in mice livers. Chapter five describes separation methods for nucleobases, nucleosides and nucleotides and their isomers by capillary electrophoresis with both uncoated silica combination techniques were employed to analyse standards of nucleobases, nucleosides, nucleotides and their isomers; and to analyse nucleic acid constituents in mice livers. Chapter six gives the conclusions and further research work in future. The relative merits of the use of LC-APCI/MS, LC-ESI/MS and CZE/MS techniques for the studies of nucleic acid constituents are compared.