Studies of nucleobases, nucleosides and nucleotides by LC, CZE, LC-MS, CZE-MS and MS techniques

This work employs RP-HPLC and CE separation techniques, soft ionisation MS techniques (ESI and APCI), and on-line LC/MS, CE/MS and MS/MS techniques to analyse both the standards and nucleic acid constituents in mice livers. Emphasis is given to the method developments and the application of these te...

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Main Author: Gong, H.
Published: Swansea University 2000
Subjects:
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637073
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spelling ndltd-bl.uk-oai-ethos.bl.uk-6370732015-03-20T05:34:20ZStudies of nucleobases, nucleosides and nucleotides by LC, CZE, LC-MS, CZE-MS and MS techniquesGong, H.2000This work employs RP-HPLC and CE separation techniques, soft ionisation MS techniques (ESI and APCI), and on-line LC/MS, CE/MS and MS/MS techniques to analyse both the standards and nucleic acid constituents in mice livers. Emphasis is given to the method developments and the application of these techniques to tackle and solve biological and chemical problem. Chapter one gives an introduction to mass spectrometry, liquid chromatography and its separation modes, and also introduces and discusses several LC/MS interfaces based on their advantages, restrictions and their applications. Chapter two introduces capillary electrophoresis, its separation modes and CE/MS interfacing techniques with discussion of both sheath-liquid based on sheathless based CE/ME interfaces. Chapter three describes the analysis of nucleobases, nucleosides and nucleotide standards and nucleic acid constituents of the mice livers by on-line LC-APCI/MS and tandem MS/MS techniques, LSQ ion-trap and TSQ 700 were used to carry out the work above. Chapter four investigates fully and characterizes nucleobases, nucleosides, nucleotides and their isomers in several solvent systems by electrospray mass spectrometry (ES/MS). Develops analytical methods with LC-ES/MS, LC/MS/MS techniques and applies these techniques to examine the standards and identify several nucleobases, nucleosides and nucleotides in mice livers. Chapter five describes separation methods for nucleobases, nucleosides and nucleotides and their isomers by capillary electrophoresis with both uncoated silica combination techniques were employed to analyse standards of nucleobases, nucleosides, nucleotides and their isomers; and to analyse nucleic acid constituents in mice livers. Chapter six gives the conclusions and further research work in future. The relative merits of the use of LC-APCI/MS, LC-ESI/MS and CZE/MS techniques for the studies of nucleic acid constituents are compared.572.072Swansea University http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637073Electronic Thesis or Dissertation
collection NDLTD
sources NDLTD
topic 572.072
spellingShingle 572.072
Gong, H.
Studies of nucleobases, nucleosides and nucleotides by LC, CZE, LC-MS, CZE-MS and MS techniques
description This work employs RP-HPLC and CE separation techniques, soft ionisation MS techniques (ESI and APCI), and on-line LC/MS, CE/MS and MS/MS techniques to analyse both the standards and nucleic acid constituents in mice livers. Emphasis is given to the method developments and the application of these techniques to tackle and solve biological and chemical problem. Chapter one gives an introduction to mass spectrometry, liquid chromatography and its separation modes, and also introduces and discusses several LC/MS interfaces based on their advantages, restrictions and their applications. Chapter two introduces capillary electrophoresis, its separation modes and CE/MS interfacing techniques with discussion of both sheath-liquid based on sheathless based CE/ME interfaces. Chapter three describes the analysis of nucleobases, nucleosides and nucleotide standards and nucleic acid constituents of the mice livers by on-line LC-APCI/MS and tandem MS/MS techniques, LSQ ion-trap and TSQ 700 were used to carry out the work above. Chapter four investigates fully and characterizes nucleobases, nucleosides, nucleotides and their isomers in several solvent systems by electrospray mass spectrometry (ES/MS). Develops analytical methods with LC-ES/MS, LC/MS/MS techniques and applies these techniques to examine the standards and identify several nucleobases, nucleosides and nucleotides in mice livers. Chapter five describes separation methods for nucleobases, nucleosides and nucleotides and their isomers by capillary electrophoresis with both uncoated silica combination techniques were employed to analyse standards of nucleobases, nucleosides, nucleotides and their isomers; and to analyse nucleic acid constituents in mice livers. Chapter six gives the conclusions and further research work in future. The relative merits of the use of LC-APCI/MS, LC-ESI/MS and CZE/MS techniques for the studies of nucleic acid constituents are compared.
author Gong, H.
author_facet Gong, H.
author_sort Gong, H.
title Studies of nucleobases, nucleosides and nucleotides by LC, CZE, LC-MS, CZE-MS and MS techniques
title_short Studies of nucleobases, nucleosides and nucleotides by LC, CZE, LC-MS, CZE-MS and MS techniques
title_full Studies of nucleobases, nucleosides and nucleotides by LC, CZE, LC-MS, CZE-MS and MS techniques
title_fullStr Studies of nucleobases, nucleosides and nucleotides by LC, CZE, LC-MS, CZE-MS and MS techniques
title_full_unstemmed Studies of nucleobases, nucleosides and nucleotides by LC, CZE, LC-MS, CZE-MS and MS techniques
title_sort studies of nucleobases, nucleosides and nucleotides by lc, cze, lc-ms, cze-ms and ms techniques
publisher Swansea University
publishDate 2000
url http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637073
work_keys_str_mv AT gongh studiesofnucleobasesnucleosidesandnucleotidesbylcczelcmsczemsandmstechniques
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