| Summary: | The main thrust of this work investigated the use of supercritical fluids in analytical chemistry. Their use for sample preparation, supercritical fluid extraction (SFE), and as a mobile phase in chromatography, supercritical fluid chromatography (SFC), was investigated for the analysis of compounds derived in the agrochemical and pharmaceutical industries. Fast, efficient separations were demonstrated with SFC for the analysis of pharmaceutical compounds and possible retention mechanisms in SFC are postulated. The hyphenation of SFE with SFC and mass spectrometry (MS) is successfully demonstrated in the analysis of a systematic fungicide from a soil matrix. Both qualitative and semi-quantitative data were obtained. A new atmospheric pressure ion source used in mass spectrometry, electrospray ionisation, was investigated for the analysis of both high and low molecular weight compounds. The technique afforded high mass measurement accuracies and the ability to be readily coupled with liquid chromatography. Experimental investigations were also made into the double ionisation energies of a series of chlorinated benzenes using double charge transfer spectrometry.
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