Enhancing the production of omega-3 polyunsaturated fatty acids in marine diatoms

The primary producers of essential omega-3 polyunsaturated fatty acids are marine microalgae, which form the base of the aquatic food web. One alternative source of our ever diminishing stocks of fish and fish oil is via the cultivation of these microorganisms. Unfortunately, these microalgae, of wh...

Full description

Bibliographic Details
Main Author: Vaezi, Royah
Published: University of East Anglia 2015
Subjects:
577
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.656120
id ndltd-bl.uk-oai-ethos.bl.uk-656120
record_format oai_dc
spelling ndltd-bl.uk-oai-ethos.bl.uk-6561202016-08-04T03:58:08ZEnhancing the production of omega-3 polyunsaturated fatty acids in marine diatomsVaezi, Royah2015The primary producers of essential omega-3 polyunsaturated fatty acids are marine microalgae, which form the base of the aquatic food web. One alternative source of our ever diminishing stocks of fish and fish oil is via the cultivation of these microorganisms. Unfortunately, these microalgae, of which diatoms are the dominant class, only accumulate oil during specific stages of their life-cycle and/or under nutritional states which are incompatible with the required high density of growth and target fatty acid profile. Additionally, the endogenous levels of desirable fatty acids such as eicospantaenoic acid (EPA; 20:5, n-3) and docosahexaenoic acid (DHA; 22:6, n-3) are usually relatively modest (in the range of 10-35% of total fatty acids) and therefore present an opportunity for enhancement. A database search carried out on the genomes of omega-3-producing unicellular photoautotrophic green alga Ostreococcus sp. RCC809 and cold-water diatom Fragilariopsis cylindrus led to the identification of four novel genes involved in omega-3 biosynthesis. These genes encoded an omega-3-specific Δ6-desaturase, a Δ4-desaturase, a Δ6-elongase and a Δ5- elongase. Overexpression of genes encoding Δ6-desaturase and Δ6-elongase activities in Thalassiosira pseudonana impacted the fatty acid and acyl-CoA profiles of this model centric diatom. Changes to chloroplast and lipid droplet phenotype were also observed. Targeted knock-down of native genes involved in the omega-3 biosynthetic pathway was carried out in T. pseudonana to further understand endogenous omega-3 fatty acid production. Cells targeted for the knock-down of Δ9-desaturase exhibited a drastically altered growth phenotype, but maintained a wild type-like fatty acid profile. This phenotype was attributed to the possibility of another, functionally redundant, protein that escaped sequence-based silencing, masking the knock-down of Δ9-desaturase. The results and observations provided in this thesis contribute new valuable information to the field of lipidomic research in microalgae, breaking new ground in metabolic engineering of lipid metabolism in diatoms.577University of East Angliahttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.656120https://ueaeprints.uea.ac.uk/53379/Electronic Thesis or Dissertation
collection NDLTD
sources NDLTD
topic 577
spellingShingle 577
Vaezi, Royah
Enhancing the production of omega-3 polyunsaturated fatty acids in marine diatoms
description The primary producers of essential omega-3 polyunsaturated fatty acids are marine microalgae, which form the base of the aquatic food web. One alternative source of our ever diminishing stocks of fish and fish oil is via the cultivation of these microorganisms. Unfortunately, these microalgae, of which diatoms are the dominant class, only accumulate oil during specific stages of their life-cycle and/or under nutritional states which are incompatible with the required high density of growth and target fatty acid profile. Additionally, the endogenous levels of desirable fatty acids such as eicospantaenoic acid (EPA; 20:5, n-3) and docosahexaenoic acid (DHA; 22:6, n-3) are usually relatively modest (in the range of 10-35% of total fatty acids) and therefore present an opportunity for enhancement. A database search carried out on the genomes of omega-3-producing unicellular photoautotrophic green alga Ostreococcus sp. RCC809 and cold-water diatom Fragilariopsis cylindrus led to the identification of four novel genes involved in omega-3 biosynthesis. These genes encoded an omega-3-specific Δ6-desaturase, a Δ4-desaturase, a Δ6-elongase and a Δ5- elongase. Overexpression of genes encoding Δ6-desaturase and Δ6-elongase activities in Thalassiosira pseudonana impacted the fatty acid and acyl-CoA profiles of this model centric diatom. Changes to chloroplast and lipid droplet phenotype were also observed. Targeted knock-down of native genes involved in the omega-3 biosynthetic pathway was carried out in T. pseudonana to further understand endogenous omega-3 fatty acid production. Cells targeted for the knock-down of Δ9-desaturase exhibited a drastically altered growth phenotype, but maintained a wild type-like fatty acid profile. This phenotype was attributed to the possibility of another, functionally redundant, protein that escaped sequence-based silencing, masking the knock-down of Δ9-desaturase. The results and observations provided in this thesis contribute new valuable information to the field of lipidomic research in microalgae, breaking new ground in metabolic engineering of lipid metabolism in diatoms.
author Vaezi, Royah
author_facet Vaezi, Royah
author_sort Vaezi, Royah
title Enhancing the production of omega-3 polyunsaturated fatty acids in marine diatoms
title_short Enhancing the production of omega-3 polyunsaturated fatty acids in marine diatoms
title_full Enhancing the production of omega-3 polyunsaturated fatty acids in marine diatoms
title_fullStr Enhancing the production of omega-3 polyunsaturated fatty acids in marine diatoms
title_full_unstemmed Enhancing the production of omega-3 polyunsaturated fatty acids in marine diatoms
title_sort enhancing the production of omega-3 polyunsaturated fatty acids in marine diatoms
publisher University of East Anglia
publishDate 2015
url http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.656120
work_keys_str_mv AT vaeziroyah enhancingtheproductionofomega3polyunsaturatedfattyacidsinmarinediatoms
_version_ 1718372419268247552