Sequencing of grass carp (ctenopharyngodon idellus) growth hormone gene and studies on its promoter activity.
by Agnes Pui-Yee Chan. === Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. === Includes bibliographical references (leaves 162-177). === ACKNOWLEDGEMENTS --- p.i === ABSTRACT --- p.ii === TABLE OF CONTENTS --- p.iv === ABBREVIATIONS --- p.vii === Chapter CHAPTER 1 --- INTRODUCTION === C...
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Chinese University of Hong Kong
1992
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ndltd-cuhk.edu.hk-oai-cuhk-dr-cuhk_3190022019-02-19T03:52:40Z Sequencing of grass carp (ctenopharyngodon idellus) growth hormone gene and studies on its promoter activity. Ctenopharyngodon idella Somatomedin Nucleotide sequence Molecular cloning by Agnes Pui-Yee Chan. Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. Includes bibliographical references (leaves 162-177). ACKNOWLEDGEMENTS --- p.i ABSTRACT --- p.ii TABLE OF CONTENTS --- p.iv ABBREVIATIONS --- p.vii Chapter CHAPTER 1 --- INTRODUCTION Chapter 1.1 --- Physiology of growth --- p.3 Chapter 1.2 --- The anterior pituitary --- p.4 Chapter 1.3 --- Chemistry of GH and the GH gene family --- p.7 Chapter 1.4 --- Biochemical effects and mode of action of GH --- p.8 Chapter 1.5 --- Control of GH at cellular level --- p.10 Chapter 1.6 --- Control of GH gene expression at molecular level Chapter 1.6.1 --- Introduction --- p.11 Chapter 1.6.2 --- Tissue-specific expression of GH gene Chapter 1.6.2.1 --- Tissue-specific transcription factors of pituitary cells --- p.20 Chapter 1.6.2.2 --- Non-tissue specific transcription factors of pituitary cells --- p.27 Chapter 1.6.2.3 --- Negatively-acting transcription factors of non-pituitary cells --- p.34 Chapter 1.6.2.4 --- Theory for tissue-specific GH gene activation --- p.39 Chapter 1.7 --- Characteristic of growth in fish --- p.40 Chapter 1.8 --- Objectives of the present study --- p.42 Chapter CHAPTER 2 --- MATERIALS AND METHODS Chapter 2.1 --- General techniques Chapter 2.1.1 --- Preparation of DNA Chapter 2.1.1.1 --- Minipreparation of DNA --- p.46 Chapter 2.1.1.2 --- Preparation of DNA using Qiagen column --- p.47 Chapter 2.1.1.3 --- Preparation of phage DNA --- p.48 Chapter 2.1.2 --- Elution of DNA from agarose gel --- p.51 Chapter 2.1.3 --- Preparation of competence cells and transformation --- p.52 Chapter 2.1.4 --- Ligation of DNA fragments --- p.53 Chapter 2.1.5 --- Cell feeding and subculturing --- p.54 Chapter 2.2 --- Special techniques Chapter 2.2.1 --- DNA sequencing --- p.56 Chapter 2.2.2 --- Polymerase chain reaction (PCR) --- p.67 Chapter 2.2.3 --- Direct sequencing of PCR products --- p.72 Chapter 2.2.4 --- Nested-deletion --- p.75 Chapter 2.2.5 --- DNA transfection --- p.81 Chapter 2.2.6 --- CAT assay --- p.86 Chapter CHAPTER 3 --- RESULTS Chapter 3.1 --- Sequencing of the grass carp GH gene Chapter 3.1.1 --- Introduction --- p.93 Chapter 3.1.2 --- Sequencing strategy --- p.94 Chapter 3.2 --- Sequence analysis of the grass carp GH gene --- p.108 Chapter 3.3 --- Functional analysis of the grass carp GH gene --- p.115 Chapter CHAPTER 4 --- DISCUSSIONS Chapter 4.1 --- DNA sequence comparison between grass carp GH gene and other organisms --- p.137 Chapter 4.2 --- Amino acid comparisons between grass carp GH and other organisms --- p.143 Chapter 4.3 --- Tissue-specific expression of GH gene Activation of transcription --- p.154 Repression of transcription --- p.155 Chapter 4.4 --- Electroporation of zebrafish eggs --- p.157 Chapter 4.5 --- Further studies --- p.160 REFERENCES --- p.162 APPENDIX --- p.178 Chinese University of Hong Kong Chan, Agnes Pui-Yee. Chinese University of Hong Kong Graduate School. Division of Biochemistry. 1992 Text bibliography print vii, 183 leaves : ill. ; 30 cm. cuhk:319002 http://library.cuhk.edu.hk/record=b5886992 eng Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) http://repository.lib.cuhk.edu.hk/en/islandora/object/cuhk%3A319002/datastream/TN/view/Sequencing%20of%20grass%20carp%20%28ctenopharyngodon%20idellus%29%20growth%20hormone%20gene%20and%20studies%20on%20its%20promoter%20activity.jpghttp://repository.lib.cuhk.edu.hk/en/item/cuhk-319002 |
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format |
Others
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Ctenopharyngodon idella Somatomedin Nucleotide sequence Molecular cloning |
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Ctenopharyngodon idella Somatomedin Nucleotide sequence Molecular cloning Sequencing of grass carp (ctenopharyngodon idellus) growth hormone gene and studies on its promoter activity. |
description |
by Agnes Pui-Yee Chan. === Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. === Includes bibliographical references (leaves 162-177). === ACKNOWLEDGEMENTS --- p.i === ABSTRACT --- p.ii === TABLE OF CONTENTS --- p.iv === ABBREVIATIONS --- p.vii === Chapter CHAPTER 1 --- INTRODUCTION === Chapter 1.1 --- Physiology of growth --- p.3 === Chapter 1.2 --- The anterior pituitary --- p.4 === Chapter 1.3 --- Chemistry of GH and the GH gene family --- p.7 === Chapter 1.4 --- Biochemical effects and mode of action of GH --- p.8 === Chapter 1.5 --- Control of GH at cellular level --- p.10 === Chapter 1.6 --- Control of GH gene expression at molecular level === Chapter 1.6.1 --- Introduction --- p.11 === Chapter 1.6.2 --- Tissue-specific expression of GH gene === Chapter 1.6.2.1 --- Tissue-specific transcription factors of pituitary cells --- p.20 === Chapter 1.6.2.2 --- Non-tissue specific transcription factors of pituitary cells --- p.27 === Chapter 1.6.2.3 --- Negatively-acting transcription factors of non-pituitary cells --- p.34 === Chapter 1.6.2.4 --- Theory for tissue-specific GH gene activation --- p.39 === Chapter 1.7 --- Characteristic of growth in fish --- p.40 === Chapter 1.8 --- Objectives of the present study --- p.42 === Chapter CHAPTER 2 --- MATERIALS AND METHODS === Chapter 2.1 --- General techniques === Chapter 2.1.1 --- Preparation of DNA === Chapter 2.1.1.1 --- Minipreparation of DNA --- p.46 === Chapter 2.1.1.2 --- Preparation of DNA using Qiagen column --- p.47 === Chapter 2.1.1.3 --- Preparation of phage DNA --- p.48 === Chapter 2.1.2 --- Elution of DNA from agarose gel --- p.51 === Chapter 2.1.3 --- Preparation of competence cells and transformation --- p.52 === Chapter 2.1.4 --- Ligation of DNA fragments --- p.53 === Chapter 2.1.5 --- Cell feeding and subculturing --- p.54 === Chapter 2.2 --- Special techniques === Chapter 2.2.1 --- DNA sequencing --- p.56 === Chapter 2.2.2 --- Polymerase chain reaction (PCR) --- p.67 === Chapter 2.2.3 --- Direct sequencing of PCR products --- p.72 === Chapter 2.2.4 --- Nested-deletion --- p.75 === Chapter 2.2.5 --- DNA transfection --- p.81 === Chapter 2.2.6 --- CAT assay --- p.86 === Chapter CHAPTER 3 --- RESULTS === Chapter 3.1 --- Sequencing of the grass carp GH gene === Chapter 3.1.1 --- Introduction --- p.93 === Chapter 3.1.2 --- Sequencing strategy --- p.94 === Chapter 3.2 --- Sequence analysis of the grass carp GH gene --- p.108 === Chapter 3.3 --- Functional analysis of the grass carp GH gene --- p.115 === Chapter CHAPTER 4 --- DISCUSSIONS === Chapter 4.1 --- DNA sequence comparison between grass carp GH gene and other organisms --- p.137 === Chapter 4.2 --- Amino acid comparisons between grass carp GH and other organisms --- p.143 === Chapter 4.3 --- Tissue-specific expression of GH gene === Activation of transcription --- p.154 === Repression of transcription --- p.155 === Chapter 4.4 --- Electroporation of zebrafish eggs --- p.157 === Chapter 4.5 --- Further studies --- p.160 === REFERENCES --- p.162 === APPENDIX --- p.178 |
author2 |
Chan, Agnes Pui-Yee. |
author_facet |
Chan, Agnes Pui-Yee. |
title |
Sequencing of grass carp (ctenopharyngodon idellus) growth hormone gene and studies on its promoter activity. |
title_short |
Sequencing of grass carp (ctenopharyngodon idellus) growth hormone gene and studies on its promoter activity. |
title_full |
Sequencing of grass carp (ctenopharyngodon idellus) growth hormone gene and studies on its promoter activity. |
title_fullStr |
Sequencing of grass carp (ctenopharyngodon idellus) growth hormone gene and studies on its promoter activity. |
title_full_unstemmed |
Sequencing of grass carp (ctenopharyngodon idellus) growth hormone gene and studies on its promoter activity. |
title_sort |
sequencing of grass carp (ctenopharyngodon idellus) growth hormone gene and studies on its promoter activity. |
publisher |
Chinese University of Hong Kong |
publishDate |
1992 |
url |
http://library.cuhk.edu.hk/record=b5886992 http://repository.lib.cuhk.edu.hk/en/item/cuhk-319002 |
_version_ |
1718979343911223296 |