Sequencing of grass carp (ctenopharyngodon idellus) growth hormone gene and studies on its promoter activity.

• Other Authors: Chan, Agnes Pui-Yee.
• Format: Others
• Language: English
• Published: Chinese University of Hong Kong 1992
• Subjects: Ctenopharyngodon idella; Somatomedin; Nucleotide sequence; Molecular cloning
• Online Access: http://library.cuhk.edu.hk/record=b5886992; http://repository.lib.cuhk.edu.hk/en/item/cuhk-319002

by Agnes Pui-Yee Chan. === Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. === Includes bibliographical references (leaves 162-177). === ACKNOWLEDGEMENTS --- p.i === ABSTRACT --- p.ii === TABLE OF CONTENTS --- p.iv === ABBREVIATIONS --- p.vii === Chapter CHAPTER 1 --- INTRODUCTION === Chapter 1.1 --- Physiology of growth --- p.3 === Chapter 1.2 --- The anterior pituitary --- p.4 === Chapter 1.3 --- Chemistry of GH and the GH gene family --- p.7 === Chapter 1.4 --- Biochemical effects and mode of action of GH --- p.8 === Chapter 1.5 --- Control of GH at cellular level --- p.10 === Chapter 1.6 --- Control of GH gene expression at molecular level === Chapter 1.6.1 --- Introduction --- p.11 === Chapter 1.6.2 --- Tissue-specific expression of GH gene === Chapter 1.6.2.1 --- Tissue-specific transcription factors of pituitary cells --- p.20 === Chapter 1.6.2.2 --- Non-tissue specific transcription factors of pituitary cells --- p.27 === Chapter 1.6.2.3 --- Negatively-acting transcription factors of non-pituitary cells --- p.34 === Chapter 1.6.2.4 --- Theory for tissue-specific GH gene activation --- p.39 === Chapter 1.7 --- Characteristic of growth in fish --- p.40 === Chapter 1.8 --- Objectives of the present study --- p.42 === Chapter CHAPTER 2 --- MATERIALS AND METHODS === Chapter 2.1 --- General techniques === Chapter 2.1.1 --- Preparation of DNA === Chapter 2.1.1.1 --- Minipreparation of DNA --- p.46 === Chapter 2.1.1.2 --- Preparation of DNA using Qiagen column --- p.47 === Chapter 2.1.1.3 --- Preparation of phage DNA --- p.48 === Chapter 2.1.2 --- Elution of DNA from agarose gel --- p.51 === Chapter 2.1.3 --- Preparation of competence cells and transformation --- p.52 === Chapter 2.1.4 --- Ligation of DNA fragments --- p.53 === Chapter 2.1.5 --- Cell feeding and subculturing --- p.54 === Chapter 2.2 --- Special techniques === Chapter 2.2.1 --- DNA sequencing --- p.56 === Chapter 2.2.2 --- Polymerase chain reaction (PCR) --- p.67 === Chapter 2.2.3 --- Direct sequencing of PCR products --- p.72 === Chapter 2.2.4 --- Nested-deletion --- p.75 === Chapter 2.2.5 --- DNA transfection --- p.81 === Chapter 2.2.6 --- CAT assay --- p.86 === Chapter CHAPTER 3 --- RESULTS === Chapter 3.1 --- Sequencing of the grass carp GH gene === Chapter 3.1.1 --- Introduction --- p.93 === Chapter 3.1.2 --- Sequencing strategy --- p.94 === Chapter 3.2 --- Sequence analysis of the grass carp GH gene --- p.108 === Chapter 3.3 --- Functional analysis of the grass carp GH gene --- p.115 === Chapter CHAPTER 4 --- DISCUSSIONS === Chapter 4.1 --- DNA sequence comparison between grass carp GH gene and other organisms --- p.137 === Chapter 4.2 --- Amino acid comparisons between grass carp GH and other organisms --- p.143 === Chapter 4.3 --- Tissue-specific expression of GH gene === Activation of transcription --- p.154 === Repression of transcription --- p.155 === Chapter 4.4 --- Electroporation of zebrafish eggs --- p.157 === Chapter 4.5 --- Further studies --- p.160 === REFERENCES --- p.162 === APPENDIX --- p.178