Preservation of urinary free catecholamines.

• Other Authors: Fung, Loi Mo.
• Format: Others
• Language: English
• Published: Chinese University of Hong Kong 1996
• Subjects: Catecholamines
• Online Access: http://library.cuhk.edu.hk/record=b5888981; http://repository.lib.cuhk.edu.hk/en/item/cuhk-321505

by Fung Loi Mo. === Thesis (M.Sc.)--Chinese University of Hong Kong, 1996. === Includes bibliographical references (leaves 78-80). === Acknowledgment --- p.1 === Summary --- p.2 === List of abbreviation --- p.4 === Index of tables --- p.6 === Index of figures --- p.7 === Chapter 1. --- Introduction --- p.9 === Chapter 2. --- Literature review === Chapter 2.1 --- Catecholamines (CATS) === Chapter 2.1.1 --- Structures and properties --- p.11 === Chapter 2.1.2 --- "Origin, biosynthesis and release" --- p.14 === Chapter 2.1.3 --- Metabolism --- p.16 === Chapter 2.1.4 --- Biological functions --- p.23 === Chapter 2.1.5 --- Significance of urinary free CATS measurement --- p.23 === Chapter 2.2 --- Methods of CATS measurement === Chapter 2.2.1 --- Colorimetric method --- p.26 === Chapter 2.2.2 --- Fluorometric method --- p.26 === Chapter 2.2.3 --- Radioenzymatic method --- p.27 === Chapter 2.2.4 --- Chromatographic method --- p.27 === Chapter 2.3 --- Method of preservation of urinary free CATS === Chapter 2.3.1 --- Reasons for preservation --- p.30 === Chapter 2.3.2 --- Common preservatives --- p.31 === Chapter 2.3.3 --- Systematic CATS preservation studies --- p.33 === Chapter 2.3.4 --- Conclusions --- p.35 === Chapter 3. --- Aim of study --- p.37 === Chapter 4. --- Materials and Methods === Chapter 4.1 --- Principle of method --- p.38 === Chapter 4.2 --- Materials --- p.39 === Chapter 4.2.1 --- Chemicals --- p.39 === Chapter 4.2.2 --- Equipment --- p.39 === Chapter 4.2.3 --- Reagent preparation for CATS analysis --- p.39 === Chapter 4.2.4 --- Reagent preparation for the different studies --- p.42 === Chapter 4.3 --- Methods === Chapter 4.3.1 --- Alumina extraction of urinary free CATS --- p.44 === Chapter 4.3.2 --- Separation and quantitation of free CATS by HPLC --- p.45 === Chapter 4.3.3 --- Statistical analysis --- p.46 === Chapter 4.4 --- Precision performance and recovery study --- p.47 === Chapter 4.5 --- Choice of buffer for preserving urinary free CATS --- p.47 === Chapter 4.6 --- Optimal concentration of citrate and formate buffer --- p.48 === Chapter 4.7 --- Effects of citrate and formate buffer on measurement of urinary free CATS --- p.50 === Chapter 4.8 --- Effects of different preservatives on urinary free CATS at 37°C --- p.52 === Chapter 4.9 --- Effects of different storage temperatures on the stability of buffered urinary free CATS --- p.52 === Chapter 5. --- Results and discussions === Chapter 5.1 --- Precision performance and recovery study --- p.56 === Chapter 5.2 --- Choice of buffer for preserving urinary free CATS --- p.58 === Chapter 5.3 --- Optimal concentration of citrate and formate buffer --- p.61 === Chapter 5.4 --- Effects of citrate and formate buffer on measurement of urinary free CATS --- p.63 === Chapter 5.5 --- Effects of different preservatives on urinary free CATS at 37°C --- p.65 === Chapter 5.6 --- Effects of different storage temperatures on the stability of buffered urinary free CATS --- p.68 === Chapter 6. --- Conclusions --- p.76 === Chapter 7. --- Limitations and future prospects --- p.77 === Chapter 8. --- References --- p.78