Influence of salinity on urea and ammonia metabolism in silver seabream (Sparus sarba).

Luk Chun-yin. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. === Includes bibliographical references (leaves 119-131). === Abstracts in English and Chinese. === ABSTRACT --- p.i === ACKNOWLEDGEMENTS --- p.iv === LIST OF FIGURES --- p.x === LIST OF TABLES --- p.xii === Chapter CHAPT...

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Other Authors: Luk, Chun-yin.
Format: Others
Language:English
Chinese
Published: 2001
Subjects:
Online Access:http://library.cuhk.edu.hk/record=b5890678
http://repository.lib.cuhk.edu.hk/en/item/cuhk-323395
id ndltd-cuhk.edu.hk-oai-cuhk-dr-cuhk_323395
record_format oai_dc
collection NDLTD
language English
Chinese
format Others
sources NDLTD
topic Sparus--Physiology
Salinity--Physiological effect
Urea--Metabolism
Ammonia--Metabolism
Fishes--Adaptation
spellingShingle Sparus--Physiology
Salinity--Physiological effect
Urea--Metabolism
Ammonia--Metabolism
Fishes--Adaptation
Influence of salinity on urea and ammonia metabolism in silver seabream (Sparus sarba).
description Luk Chun-yin. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. === Includes bibliographical references (leaves 119-131). === Abstracts in English and Chinese. === ABSTRACT --- p.i === ACKNOWLEDGEMENTS --- p.iv === LIST OF FIGURES --- p.x === LIST OF TABLES --- p.xii === Chapter CHAPTER 1 --- GENERAL INTRODUCTION --- p.1 === Chapter CHAPTER 2 --- LITERATURE REVIEW --- p.6 === Chapter 2.1 --- Introduction --- p.7 === Chapter 2.2 --- Ammonia chemistry --- p.10 === Chapter 2.3 --- Ammonia metabolism and excretion --- p.11 === Chapter 2.3.1 --- Ammonia production --- p.11 === Chapter 2.3.2 --- Blood levels of ammonia --- p.12 === Chapter 2.3.3 --- Ammonia Excretion --- p.17 === Chapter 2.4 --- Urea metabolism and excretion --- p.23 === Chapter 2.4.1 --- Urea Chemistry --- p.23 === Chapter 2.4.2 --- Urea production in fishes --- p.24 === Chapter 2.4.3 --- Argininolysis --- p.25 === Chapter 2.4.4 --- Uricolysis --- p.26 === Chapter 2.4.5 --- Ornithine-urea Cycle (OUC) --- p.28 === Chapter 2.4.5.1 --- Tilapia inhabiting the highly alkaline Lake Magadi --- p.32 === Chapter 2.4.5.2 --- High Ambient Ammonia --- p.33 === Chapter 2.4.5.3 --- Air Exposure --- p.34 === Chapter 2.4.5.4 --- Toadfishes --- p.34 === Chapter 2.4.6 --- Blood urea concentration --- p.35 === Chapter 2.4.7 --- Urea excretion in fishes --- p.37 === Chapter 2.4.7.1 --- Branchial urea excretion in fishes --- p.37 === Chapter 2.4.7.2 --- Mechanisms of renal excretion in fishes --- p.40 === Chapter 2.5 --- Influence of environmental salinity on nitrogen excretion in teleosts --- p.42 === Chapter CHAPTER 3 --- BODY COMPOSITION AND UREA BIOSYNTHESIS OF SPAR US SARBA IN DIFFERENT SALINITIES --- p.46 === Chapter 3.1 --- Introduction --- p.47 === Chapter 3.2 --- Materials and Methods --- p.49 === Chapter 3.2.1 --- Experimental animals --- p.49 === Chapter 3.2.2 --- Tissue sampling --- p.49 === Chapter 3.2.3 --- Water chemistry analysis --- p.50 === Chapter 3.2.4 --- Hematological parameters --- p.50 === Chapter 3.2.5 --- Metabolite and electrolyte contents --- p.51 === Chapter 3.2.6 --- Hepatic enzymes activities --- p.51 === Chapter 3.2.6.1 --- Tissue preparation --- p.51 === Chapter 3.2.6.2 --- Carbamyl phosphate synthetases (CPSases; E.C. 2.7.2.5) --- p.52 === Chapter 3.2.6.3 --- Ornithine carbamoyl transferase (OCTase; E.C. 2.1.3.3) --- p.53 === Chapter 3.2.6.4 --- Argininosuccinate synthetase (ASS; E.C. 6.3.4.5) --- p.54 === Chapter 3.2.6.5 --- Argininosuccinate lyase (ASL; E.C. 4.3.2.1) --- p.54 === Chapter 3.2.6.6 --- Arginase (ARG; 3.5.3.1) --- p.55 === Chapter 3.2.6.7 --- Glutamate dehydrogenase (EC 1.4.1.3) --- p.55 === Chapter 3.2.6.8 --- Uricase (E.C. 1.7.3.3) --- p.56 === Chapter 3.2.6.9 --- Allantoinase --- p.57 === Chapter 3.2.6.10 --- Allantoicase --- p.57 === Chapter 3.2.7 --- Statistical analysis --- p.58 === Chapter 3.3 --- Results --- p.59 === Chapter 3.3.1 --- "Changes in hepatosmatic index, renal somatic index, muscle water and lipid content and hematological parametersin response to different salinity acclimation" --- p.59 === Chapter 3.3.2 --- Changes in serum chemistry in response to different salinity acclimation --- p.60 === Chapter 3.3.3 --- Changes in hepatic ornithine-urea cycle enzyme activitiesin response to different salinity acclimation --- p.61 === Chapter 3.3.4 --- Changes in GDHase and uricolytic enzyme activitiesin response to different salinity acclimation --- p.62 === Chapter 3.4 --- Discussion --- p.71 === Chapter 3.4.1 --- Hematological responses --- p.72 === Chapter 3.4.2 --- Muscle moisture content --- p.74 === Chapter 3.4.3 --- Circulating electrolyte levels --- p.75 === Chapter 3.4.4 --- Circulating metabolites levels --- p.77 === Chapter 3.4.5 --- Urea metabolism --- p.80 === Chapter 3.4.5.1 --- Ornithine-urea cycle enzymes --- p.80 === Chapter 3.4.5.2 --- Carbamoyl phosphate synthetase isozymes --- p.81 === Chapter 3.4.5.3 --- Uricolytic pathway and argininolysis --- p.85 === Chapter 3.4.5.4 --- Influence of salinity on urea metabolism --- p.86 === Chapter 3.4.6 --- Conclusion --- p.87 === Chapter CHAPTER 4 --- EFFECT OF SALINITY ON NITROGEN EXCRETION OF SPARUS SARBA --- p.88 === Chapter 4.1 --- Introduction --- p.89 === Chapter 4.2 --- Materials and Methods --- p.91 === Chapter 4.2.1 --- Experimental animals --- p.91 === Chapter 4.2.2 --- Experimental protocol --- p.92 === Chapter 4.2.3 --- Determination of net ammonia and urea excretion rates --- p.94 === Chapter 4.2.4 --- Statistical analysis --- p.94 === Chapter 4.3 --- Results --- p.95 === Chapter 4.3.1 --- Net ammonia-N and urea-N excretion rates --- p.95 === Chapter 4.3.2 --- Changes in net ammonia-N and urea-N excretion ratesin response to abrupt hyposmotic exposure --- p.95 === Chapter 4.3.3 --- Changes in net ammonia-N and urea-N excretion rates after exposure to amiloride for 3 hours --- p.96 === Chapter 4.3.4 --- Changes in net urea-N excretion rates in response to elevated body urea levels --- p.96 === Chapter 4.3.5 --- Changes in net ammonia-N excretion rates in response to elevated body ammonia levels --- p.97 === Chapter 4.4 --- Discussion --- p.106 === Chapter 4.4.1 --- Influence of environmental salinity on net ammonia-N and urea-N excretion rates --- p.106 === Chapter 4.4.2 --- Effects of amiloride on nitrogen excretion --- p.109 === Chapter 4.4.3 --- Effect of increased body ammonia on ammonia excretion --- p.113 === Chapter 4.4.4 --- Changes in net urea-N excretion rates in response to elevated body urea levels --- p.113 === Chapter 4.5 --- Conclusion --- p.114 === Chapter CHAPTER 5 --- GENERAL CONCLUSION --- p.115 === references --- p.119
author2 Luk, Chun-yin.
author_facet Luk, Chun-yin.
title Influence of salinity on urea and ammonia metabolism in silver seabream (Sparus sarba).
title_short Influence of salinity on urea and ammonia metabolism in silver seabream (Sparus sarba).
title_full Influence of salinity on urea and ammonia metabolism in silver seabream (Sparus sarba).
title_fullStr Influence of salinity on urea and ammonia metabolism in silver seabream (Sparus sarba).
title_full_unstemmed Influence of salinity on urea and ammonia metabolism in silver seabream (Sparus sarba).
title_sort influence of salinity on urea and ammonia metabolism in silver seabream (sparus sarba).
publishDate 2001
url http://library.cuhk.edu.hk/record=b5890678
http://repository.lib.cuhk.edu.hk/en/item/cuhk-323395
_version_ 1718982772776763392
spelling ndltd-cuhk.edu.hk-oai-cuhk-dr-cuhk_3233952019-02-26T03:34:30Z Influence of salinity on urea and ammonia metabolism in silver seabream (Sparus sarba). Sparus--Physiology Salinity--Physiological effect Urea--Metabolism Ammonia--Metabolism Fishes--Adaptation Luk Chun-yin. Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. Includes bibliographical references (leaves 119-131). Abstracts in English and Chinese. ABSTRACT --- p.i ACKNOWLEDGEMENTS --- p.iv LIST OF FIGURES --- p.x LIST OF TABLES --- p.xii Chapter CHAPTER 1 --- GENERAL INTRODUCTION --- p.1 Chapter CHAPTER 2 --- LITERATURE REVIEW --- p.6 Chapter 2.1 --- Introduction --- p.7 Chapter 2.2 --- Ammonia chemistry --- p.10 Chapter 2.3 --- Ammonia metabolism and excretion --- p.11 Chapter 2.3.1 --- Ammonia production --- p.11 Chapter 2.3.2 --- Blood levels of ammonia --- p.12 Chapter 2.3.3 --- Ammonia Excretion --- p.17 Chapter 2.4 --- Urea metabolism and excretion --- p.23 Chapter 2.4.1 --- Urea Chemistry --- p.23 Chapter 2.4.2 --- Urea production in fishes --- p.24 Chapter 2.4.3 --- Argininolysis --- p.25 Chapter 2.4.4 --- Uricolysis --- p.26 Chapter 2.4.5 --- Ornithine-urea Cycle (OUC) --- p.28 Chapter 2.4.5.1 --- Tilapia inhabiting the highly alkaline Lake Magadi --- p.32 Chapter 2.4.5.2 --- High Ambient Ammonia --- p.33 Chapter 2.4.5.3 --- Air Exposure --- p.34 Chapter 2.4.5.4 --- Toadfishes --- p.34 Chapter 2.4.6 --- Blood urea concentration --- p.35 Chapter 2.4.7 --- Urea excretion in fishes --- p.37 Chapter 2.4.7.1 --- Branchial urea excretion in fishes --- p.37 Chapter 2.4.7.2 --- Mechanisms of renal excretion in fishes --- p.40 Chapter 2.5 --- Influence of environmental salinity on nitrogen excretion in teleosts --- p.42 Chapter CHAPTER 3 --- BODY COMPOSITION AND UREA BIOSYNTHESIS OF SPAR US SARBA IN DIFFERENT SALINITIES --- p.46 Chapter 3.1 --- Introduction --- p.47 Chapter 3.2 --- Materials and Methods --- p.49 Chapter 3.2.1 --- Experimental animals --- p.49 Chapter 3.2.2 --- Tissue sampling --- p.49 Chapter 3.2.3 --- Water chemistry analysis --- p.50 Chapter 3.2.4 --- Hematological parameters --- p.50 Chapter 3.2.5 --- Metabolite and electrolyte contents --- p.51 Chapter 3.2.6 --- Hepatic enzymes activities --- p.51 Chapter 3.2.6.1 --- Tissue preparation --- p.51 Chapter 3.2.6.2 --- Carbamyl phosphate synthetases (CPSases; E.C. 2.7.2.5) --- p.52 Chapter 3.2.6.3 --- Ornithine carbamoyl transferase (OCTase; E.C. 2.1.3.3) --- p.53 Chapter 3.2.6.4 --- Argininosuccinate synthetase (ASS; E.C. 6.3.4.5) --- p.54 Chapter 3.2.6.5 --- Argininosuccinate lyase (ASL; E.C. 4.3.2.1) --- p.54 Chapter 3.2.6.6 --- Arginase (ARG; 3.5.3.1) --- p.55 Chapter 3.2.6.7 --- Glutamate dehydrogenase (EC 1.4.1.3) --- p.55 Chapter 3.2.6.8 --- Uricase (E.C. 1.7.3.3) --- p.56 Chapter 3.2.6.9 --- Allantoinase --- p.57 Chapter 3.2.6.10 --- Allantoicase --- p.57 Chapter 3.2.7 --- Statistical analysis --- p.58 Chapter 3.3 --- Results --- p.59 Chapter 3.3.1 --- "Changes in hepatosmatic index, renal somatic index, muscle water and lipid content and hematological parametersin response to different salinity acclimation" --- p.59 Chapter 3.3.2 --- Changes in serum chemistry in response to different salinity acclimation --- p.60 Chapter 3.3.3 --- Changes in hepatic ornithine-urea cycle enzyme activitiesin response to different salinity acclimation --- p.61 Chapter 3.3.4 --- Changes in GDHase and uricolytic enzyme activitiesin response to different salinity acclimation --- p.62 Chapter 3.4 --- Discussion --- p.71 Chapter 3.4.1 --- Hematological responses --- p.72 Chapter 3.4.2 --- Muscle moisture content --- p.74 Chapter 3.4.3 --- Circulating electrolyte levels --- p.75 Chapter 3.4.4 --- Circulating metabolites levels --- p.77 Chapter 3.4.5 --- Urea metabolism --- p.80 Chapter 3.4.5.1 --- Ornithine-urea cycle enzymes --- p.80 Chapter 3.4.5.2 --- Carbamoyl phosphate synthetase isozymes --- p.81 Chapter 3.4.5.3 --- Uricolytic pathway and argininolysis --- p.85 Chapter 3.4.5.4 --- Influence of salinity on urea metabolism --- p.86 Chapter 3.4.6 --- Conclusion --- p.87 Chapter CHAPTER 4 --- EFFECT OF SALINITY ON NITROGEN EXCRETION OF SPARUS SARBA --- p.88 Chapter 4.1 --- Introduction --- p.89 Chapter 4.2 --- Materials and Methods --- p.91 Chapter 4.2.1 --- Experimental animals --- p.91 Chapter 4.2.2 --- Experimental protocol --- p.92 Chapter 4.2.3 --- Determination of net ammonia and urea excretion rates --- p.94 Chapter 4.2.4 --- Statistical analysis --- p.94 Chapter 4.3 --- Results --- p.95 Chapter 4.3.1 --- Net ammonia-N and urea-N excretion rates --- p.95 Chapter 4.3.2 --- Changes in net ammonia-N and urea-N excretion ratesin response to abrupt hyposmotic exposure --- p.95 Chapter 4.3.3 --- Changes in net ammonia-N and urea-N excretion rates after exposure to amiloride for 3 hours --- p.96 Chapter 4.3.4 --- Changes in net urea-N excretion rates in response to elevated body urea levels --- p.96 Chapter 4.3.5 --- Changes in net ammonia-N excretion rates in response to elevated body ammonia levels --- p.97 Chapter 4.4 --- Discussion --- p.106 Chapter 4.4.1 --- Influence of environmental salinity on net ammonia-N and urea-N excretion rates --- p.106 Chapter 4.4.2 --- Effects of amiloride on nitrogen excretion --- p.109 Chapter 4.4.3 --- Effect of increased body ammonia on ammonia excretion --- p.113 Chapter 4.4.4 --- Changes in net urea-N excretion rates in response to elevated body urea levels --- p.113 Chapter 4.5 --- Conclusion --- p.114 Chapter CHAPTER 5 --- GENERAL CONCLUSION --- p.115 references --- p.119 Luk, Chun-yin. Chinese University of Hong Kong Graduate School. Division of Biology. 2001 Text bibliography print xiii, 131 leaves : ill. ; 30 cm. cuhk:323395 http://library.cuhk.edu.hk/record=b5890678 eng chi Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) http://repository.lib.cuhk.edu.hk/en/islandora/object/cuhk%3A323395/datastream/TN/view/Influence%20of%20salinity%20on%20urea%20and%20ammonia%20metabolism%20in%20silver%20seabream%20%28Sparus%20sarba%29.jpghttp://repository.lib.cuhk.edu.hk/en/item/cuhk-323395