Epigenetic alterations in endometrial cancer and it's precursors.

• Other Authors: Cheung, Ka Wai.
• Format: Others
• Language: English; Chinese
• Published: 2004
• Subjects: Endometrium > Cancer; Endometrial Neoplasms; Carcinoma > pathology
• Online Access: http://library.cuhk.edu.hk/record=b5891995; http://repository.lib.cuhk.edu.hk/en/item/cuhk-324734

Cheung Ka Wai. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. === Includes bibliographical references (leaves 83-93). === Abstracts in English and Chinese. === Acknowledgments --- p.i === Publications --- p.ii === Awards --- p.iii === List of abbreviations --- p.iv === List of figures --- p.vi === List of tables --- p.vii === Abstract in English --- p.viii === Abstract in Chinese --- p.ix === Table of Contents === Chapter Chapter 1 --- Introduction --- p.1 === Chapter Chapter 2 --- literature Review === Chapter 2.1 --- Anatomy and Physiology of Endometrium --- p.2 === Chapter 2.2 --- Endometrial cancer --- p.5 === Chapter 2.2.1 --- Epidemiology --- p.6 === Chapter 2.2.2 --- Etiologies and Risk Factors --- p.7 === Chapter 2.3 --- Pathology --- p.11 === Chapter 2.3.1 --- Grading of endometrial cancer --- p.14 === Chapter 2.3.2 --- Staging of endometrial cancer --- p.14 === Chapter 2.4 --- Prevention and Treatment --- p.16 === Chapter 2.5 --- Molecular alterations in endometrial cancer --- p.17 === Chapter 2.5.1 --- Genetic alterations in endometrial cancer --- p.18 === Chapter 2.5.1.1 --- Oncogene activation --- p.19 === Chapter 2.5.1.2 --- Tumor suppressor gene inactivation --- p.20 === Chapter 2.5.1.2.1 --- Mutation and loss of heterozygosity of tumor suppressor genes in endometrial cancer --- p.22 === Chapter 2.5.2 --- Epigenetic alterations --- p.27 === Chapter 2.5.2.1 --- CpG islands methylation --- p.29 === Chapter 2.5.2.2 --- de novo methylation --- p.29 === Chapter 2.5.2.3 --- Detection of gene promoter hypermethylation --- p.31 === Chapter 2.5.2.4 --- Epigenetic alteration in endometrial cancer --- p.31 === Chapter 2.5.2.5 --- Promoter hypermethylation of tumor suppressor genes in other cancers --- p.39 === Chapter 2.5.3 --- Microsatellite instability --- p.42 === Chapter Chapter 3 --- The objectives of study --- p.45 === Chapter Chapter 4 --- Materials and Methods --- p.46 === Chapter 4.1 --- Samples --- p.46 === Chapter 4.1.1 --- Formalin fixed paraffin embedded tissues --- p.46 === Chapter 4.1.2 --- Cell lines --- p.46 === Chapter 4.2 --- Histological grading and staging of samples --- p.47 === Chapter 4.3 --- Microdissection on tissue sections --- p.47 === Chapter 4.4 --- Extraction of nucleic acid === Chapter 4.4.1 --- Extraction of DNA from paraffin-embedded tissues --- p.48 === Chapter 4.4.2 --- Extraction of DNA from cell lines --- p.49 === Chapter 4.5 --- DNA methylation analysis --- p.49 === Chapter 4.5.1 --- Overview of Methylation-Specific PCR (MSP) --- p.49 === Chapter 4.5.2 --- Bisulfite modification of DNA --- p.50 === Chapter 4.5.3 --- Methylation specific PCR (MSP) --- p.51 === Chapter 4.6 --- Microsatellite Analysis --- p.53 === Chapter 4.7 --- Statistical analysis --- p.56 === Chapter Chapter 5 --- Results === Chapter 5.1 --- Clinical-pathological features of endometrioid adenocarcinoma --- p.57 === Chapter 5.2 --- Promoter hypermethylation in endometrial cancer --- p.57 === Chapter 5.3 --- Microsatellite status (MSI) analysis --- p.65 === Chapter Chapter 6 --- Discussion === Chapter 6.1 --- Promoter hypermethylation in endometrial cancer --- p.71 === Chapter 6.1.1 --- Concurrent hypermethylation of multiple genesin endometrioid adenocarcinoma and its precursor lesions --- p.72 === Chapter 6.1.1.1 --- Promoter hypermethylation of E-cad --- p.73 === Chapter 6.1.1.2 --- Promoter hypermethylation of APC --- p.73 === Chapter 6.1.1.3 --- Promoter hypermethylation of MGMT --- p.74 === Chapter 6.1.1.4 --- Promoter hypermethylation of RASSF1A --- p.75 === Chapter 6.1.1.5 --- Promoter hypermethylation of hMLH-1 --- p.76 === Chapter 6.1.1.6 --- Promoter hypermethylation in ECA coexisting with hyperplasia and not coexisting with hyperplasia --- p.77 === Chapter 6.1.2 --- Promoter hypermethylation in SCA --- p.77 === Chapter 6.2 --- Microsatellite status analysis --- p.78 === Chapter 6.2.1 --- MSI in endometrial cancer --- p.78 === Chapter 6.2.2 --- MSI and concurrent promoter hypermethylation --- p.79 === Chapter 6.2.3 --- MSI and promoter hypermethylation of hMLH-1 --- p.80 === Chapter Chapter 7 --- Conclusion --- p.81 === Further studies --- p.82 === References --- p.83