Mechanisms for stimulation of C1- secretion by scutellariae radix extract and its major flavonoid baicalein in human colonic T84 cells.

• Other Authors: Yip, Wai Nga.
• Format: Others
• Language: English; Chinese
• Published: 2004
• Subjects: Flavonoids; Colon (Anatomy) > Effect of drugs on; Scutellaria baicalensis; Colon > drug effects
• Online Access: http://library.cuhk.edu.hk/record=b5892078; http://repository.lib.cuhk.edu.hk/en/item/cuhk-324799

Yip Wai Nga. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. === Includes bibliographical references (leaves 93-101). === Abstracts in English and Chinese. === Abstract (English version) --- p.i === Abstract (Chinese version) --- p.v === Acknowledgements --- p.viii === Table of contents --- p.ix === List of figures --- p.xii === List of abbreviation --- p.xv === Chapter Chapter I: --- Introduction --- p.1 === Chapter I.1 --- Transepithelial ion transport --- p.1 === Chapter I.1.1 --- Fluid secretion in colon --- p.1 === Chapter I.1.2. --- Cellular mechanism of chloride secretion --- p.3 === Chapter 1.2. --- Regulation of chloride secretion in T84 cells --- p.6 === Chapter I.2.1 --- Human colonic T84 cells as the study model --- p.6 === Chapter I.2.2 --- Signal transduction pathways of chloride secretion in T84 cells --- p.13 === Chapter I.3. --- Pharmacological actions of Scutellariae Radix --- p.13 === Chapter I.3.1. --- What is Scutellariae Radix? --- p.13 === Chapter I.3.2. --- Some biological and pharmacological actions of Scutellariae Radix --- p.13 === Chapter I.4. --- Effects of Scutellariae Radix and its major flavonoid baicalein on ion transport in T84 cells --- p.15 === Chapter I.4.1. --- Effects of Scutellariae Radix extract on ion transport in T84 cells --- p.15 === Chapter I.4.2. --- Biological effects of baicalein --- p.15 === Chapter I.5 --- "Relationship of Coptidis rhizoma and its active ingredient berberine, with Scutellariae radix in traditional remedies" --- p.18 === Chapter I.6 --- Aim of study --- p.20 === Chapter Chapter II: --- Methods and Materials --- p.21 === Chapter II.1. --- Culture technique of the T84 cells --- p.21 === Chapter II.2. --- Conventional short-circuit current (Isc) measurement --- p.24 === Chapter II.2.1. --- Experimental setup --- p.24 === Chapter II.2.2. --- Preparation of the permeable supports --- p.27 === Chapter II.2.3. --- Cell seeding --- p.27 === Chapter II.2.4. --- Short-circuit measurement --- p.29 === Chapter II.2.5 --- Short-circuit measurement in nystatin-permeabilized T84 monolayers --- p.30 === Chapter II.3. --- Measurement of protein kinase A activity --- p.31 === Chapter II.4. --- Solutions and chemicals --- p.32 === Chapter II.5. --- Statistical analysis --- p.33 === Chapter Chapter III: --- Result --- p.34 === Chapter III.1. --- Effect of SRE on transepithelial ion transport processes in T84 monolayers --- p.35 === Chapter III.1.1 --- Effect of SRE and baicalein on baseline Isc --- p.35 === Chapter III.1.2 --- Effect of ion channel blockers on SRE-stimulated Isc --- p.39 === Chapter III.1.3 --- Effect of K+ channel blockers on SRE-stimulated Isc --- p.42 === Chapter III.1.4 --- Effect of SRE in C1- free solution --- p.48 === Chapter III.2. --- Effect of SRE on apical C1- conductance and basolateral K+ conductance in nystatin-permeabilized T84 monolayers --- p.51 === Chapter III.2.1 --- Effect of SRE and baicalein on baseline IC1 --- p.51 === Chapter III.2.2 --- Study of apical C1- conductance in T84 monolayers --- p.54 === Chapter III.2.3 --- Interaction of SRE and forskolin --- p.61 === Chapter III.2.4 --- Study of basolateral K+ conductance in T84 monolayers --- p.62 === Chapter III.3 --- Effect of SRE and baicalein on PKA activities in T84 cells --- p.64 === Chapter III.3.1 --- Effect of Scutellariae Radix on PKA activity --- p.66 === Chapter III.3.2 --- Effect of baicalein on PKA activity --- p.69 === Chapter III.3.3. --- Effect of berberine on PKA activity --- p.69 === Chapter III.3.3. --- Interaction of baicalein and berberine on PKA activity --- p.74 === Chapter Chapter IV: --- Discussion --- p.77 === Chapter IV.1 --- "Scutellariae Radix，Coptidis Rhizoma, and gastrointestinal secretory function" --- p.77 === Chapter IV.2 --- SRE- and baicalein-induced increase in Isc --- p.79 === Chapter IV.3 --- Cellular signaling mechanisms underlying the effect of SRE and baicalein --- p.82 === Chapter IV.4 --- "Interaction between Scutellariae Radix and Coptidis Rhizoma - the ""ying and yang"" hypothesis" --- p.89 === Chapter IV.5 --- Summary --- p.91