Growth inhibitory effects of chlorophyllin on human breast carcinoma MCF-7 cells.
Kong Ka-lai. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. === Includes bibliographical references (leaves 126-149). === Abstracts in English and Chinese. === Acknowledgements --- p.i === Abstract --- p.ii === Abstract (Chinese Version) --- p.vi === Table of Contents --- p.ix ===...
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2005
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Online Access: | http://library.cuhk.edu.hk/record=b5892491 http://repository.lib.cuhk.edu.hk/en/item/cuhk-325348 |
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English Chinese |
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topic |
Chlorophyllin Antineoplastic agents Breast--Cancer--Chemoprevention Chlorophyllides Antineoplastic Agents Breast Neoplasms--drug therapy |
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Chlorophyllin Antineoplastic agents Breast--Cancer--Chemoprevention Chlorophyllides Antineoplastic Agents Breast Neoplasms--drug therapy Growth inhibitory effects of chlorophyllin on human breast carcinoma MCF-7 cells. |
description |
Kong Ka-lai. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. === Includes bibliographical references (leaves 126-149). === Abstracts in English and Chinese. === Acknowledgements --- p.i === Abstract --- p.ii === Abstract (Chinese Version) --- p.vi === Table of Contents --- p.ix === List of Figures/Table --- p.xiii === List of Abbreviations --- p.xvi === Chapter Chapter 1 --- General Introduction === Chapter 1.1 --- An Overview on Cancer --- p.1 === Chapter 1.2 --- Biological Effects of Chlorophyllin --- p.7 === Chapter 1.2.1 --- CHL as Photosensitizer --- p.7 === Chapter 1.2.2 --- CHL as Antioxidant --- p.8 === Chapter 1.2.3 --- CHL as Anticarcinogenic Agent --- p.9 === Chapter 1.3 --- Regulation of Cell Cycle --- p.13 === Chapter 1.3.1 --- Cell-Cycle Checkpoints --- p.13 === Chapter 1.3.2 --- Cell-Cycle Regulatory Proteins --- p.15 === Chapter 1.4 --- Regulation of Mitogen-Activated Protein Kinase (MAPK) Signaling Cascade --- p.21 === Chapter 1.5 --- Programmed Cell Death (or Apoptosis) --- p.27 === Chapter 1.5.1 --- Regulation of Caspase-Dependent Apoptosis --- p.28 === Chapter 1.5.2 --- Regulation of Caspase-Independent Cell Death --- p.32 === Chapter 1.5.3 --- Bcl-2 Family Proteins in Modulation of Cell Death --- p.32 === Chapter 1.6 --- In Vivo Antitumor Screening System --- p.37 === Chapter 1.7 --- Aims of the Present Study --- p.38 === Chapter Chapter 2 --- In Vitro Studies of the Anticancer Effect of Chlorophyllin === Chapter 2.1 --- Introduction --- p.39 === Chapter 2.1.1 --- DNA-Flow Cytometric Analysis --- p.51 === Chapter 2.1.2 --- Western Blot Analysis --- p.54 === Chapter 2.2 --- Materials and Methods --- p.56 === Chapter 2.2.1 --- Maintenance of Cell Lines --- p.56 === Chapter 2.2.2 --- Cytotoxic and Cytostatic Effects on the Cancer Cells --- p.56 === Chapter 2.2.3 --- DNA-Flow Cytometric Analysis --- p.60 === Chapter 2.2.4 --- Western Blot Analysis --- p.61 === Chapter 2.2.5 --- JC-1 Mitochondrial Potential Sensor --- p.64 === Chapter 2.2.6 --- Caspase Inhibitors --- p.65 === Chapter 2.2.7 --- Statistical Analysis --- p.66 === Chapter 2.2.8 --- Densitometric Analysis --- p.66 === Chapter 2.3 --- Results --- p.67 === Chapter 2.3.1 --- Effects of CHL on the Growth of Human Cancer Cells by MTT Assay --- p.67 === Chapter 2.3.2 --- Effect of CHL on the Proliferation of MCF-7 Cells by Chemi-BrdU Incorporation --- p.69 === Chapter 2.3.3 --- Effect of CHL on Cell Cycle of MCF-7 Cells --- p.71 === Chapter 2.3.4 --- Effect of CHL on the Cyclin D1 Expression in MCF-7 Cells --- p.74 === Chapter 2.3.5 --- Effects of CHL on JNK and c-Jun Expressions and Their Phosphorylations in MCF-7 Cells --- p.76 === Chapter 2.3.6 --- Effect of CHL on DNA fragmentation in MCF-7 Cells --- p.78 === Chapter 2.3.7 --- Effect of CHL on Mitochondrial Membrane Potential of MCF-7 Cells --- p.80 === Chapter 2.3.8 --- Effects of CHL on the PARP Expression and Cleavage in MCF-7 Cells --- p.83 === Chapter 2.3.9 --- "Effects of CHL on Bcl-2, Bcl-xL and Bad Expressions in MCF-7 Cells" --- p.85 === Chapter 2.3.10 --- Effects of CHL on Caspase Activations in MCF-7 Cells --- p.88 === Chapter 2.3.11 --- Effects of Caspase Inhibitors on the CHL-Induced Apoptosis in MCF-7 Cells --- p.90 === Chapter 2.4 --- Discussion --- p.93 === Chapter Chapter 3 --- In Vivo Studies of the Anticancer Effect of Chlorophyllin === Chapter 3.1 --- Introduction --- p.104 === Chapter 3.2 --- Materials and Methods --- p.106 === Chapter 3.2.1 --- Transplantation of MCF-7 Cells into the Nude Mice and Treatment --- p.106 === Chapter 3.2.2 --- Western Blot Analysis --- p.107 === Chapter 3.2.3 --- Statistical Analysis --- p.107 === Chapter 3.3 --- Results --- p.108 === Chapter 3.3.1 --- In Vivo Antitumor Activity of CHL --- p.108 === Chapter 3.3.2 --- In Vivo Effects of CHL on Cyclin D1 and Bcl-2 Expressions in MCF-7 Solid Tumor --- p.111 === Chapter 3.4 --- Discussion --- p.113 === Chapter Chapter 4 --- General Discussion --- p.115 === References --- p.126 |
author2 |
Kong, Ka-lai. |
author_facet |
Kong, Ka-lai. |
title |
Growth inhibitory effects of chlorophyllin on human breast carcinoma MCF-7 cells. |
title_short |
Growth inhibitory effects of chlorophyllin on human breast carcinoma MCF-7 cells. |
title_full |
Growth inhibitory effects of chlorophyllin on human breast carcinoma MCF-7 cells. |
title_fullStr |
Growth inhibitory effects of chlorophyllin on human breast carcinoma MCF-7 cells. |
title_full_unstemmed |
Growth inhibitory effects of chlorophyllin on human breast carcinoma MCF-7 cells. |
title_sort |
growth inhibitory effects of chlorophyllin on human breast carcinoma mcf-7 cells. |
publishDate |
2005 |
url |
http://library.cuhk.edu.hk/record=b5892491 http://repository.lib.cuhk.edu.hk/en/item/cuhk-325348 |
_version_ |
1718990315968266240 |
spelling |
ndltd-cuhk.edu.hk-oai-cuhk-dr-cuhk_3253482019-03-05T03:34:34Z Growth inhibitory effects of chlorophyllin on human breast carcinoma MCF-7 cells. Chlorophyllin Antineoplastic agents Breast--Cancer--Chemoprevention Chlorophyllides Antineoplastic Agents Breast Neoplasms--drug therapy Kong Ka-lai. Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. Includes bibliographical references (leaves 126-149). Abstracts in English and Chinese. Acknowledgements --- p.i Abstract --- p.ii Abstract (Chinese Version) --- p.vi Table of Contents --- p.ix List of Figures/Table --- p.xiii List of Abbreviations --- p.xvi Chapter Chapter 1 --- General Introduction Chapter 1.1 --- An Overview on Cancer --- p.1 Chapter 1.2 --- Biological Effects of Chlorophyllin --- p.7 Chapter 1.2.1 --- CHL as Photosensitizer --- p.7 Chapter 1.2.2 --- CHL as Antioxidant --- p.8 Chapter 1.2.3 --- CHL as Anticarcinogenic Agent --- p.9 Chapter 1.3 --- Regulation of Cell Cycle --- p.13 Chapter 1.3.1 --- Cell-Cycle Checkpoints --- p.13 Chapter 1.3.2 --- Cell-Cycle Regulatory Proteins --- p.15 Chapter 1.4 --- Regulation of Mitogen-Activated Protein Kinase (MAPK) Signaling Cascade --- p.21 Chapter 1.5 --- Programmed Cell Death (or Apoptosis) --- p.27 Chapter 1.5.1 --- Regulation of Caspase-Dependent Apoptosis --- p.28 Chapter 1.5.2 --- Regulation of Caspase-Independent Cell Death --- p.32 Chapter 1.5.3 --- Bcl-2 Family Proteins in Modulation of Cell Death --- p.32 Chapter 1.6 --- In Vivo Antitumor Screening System --- p.37 Chapter 1.7 --- Aims of the Present Study --- p.38 Chapter Chapter 2 --- In Vitro Studies of the Anticancer Effect of Chlorophyllin Chapter 2.1 --- Introduction --- p.39 Chapter 2.1.1 --- DNA-Flow Cytometric Analysis --- p.51 Chapter 2.1.2 --- Western Blot Analysis --- p.54 Chapter 2.2 --- Materials and Methods --- p.56 Chapter 2.2.1 --- Maintenance of Cell Lines --- p.56 Chapter 2.2.2 --- Cytotoxic and Cytostatic Effects on the Cancer Cells --- p.56 Chapter 2.2.3 --- DNA-Flow Cytometric Analysis --- p.60 Chapter 2.2.4 --- Western Blot Analysis --- p.61 Chapter 2.2.5 --- JC-1 Mitochondrial Potential Sensor --- p.64 Chapter 2.2.6 --- Caspase Inhibitors --- p.65 Chapter 2.2.7 --- Statistical Analysis --- p.66 Chapter 2.2.8 --- Densitometric Analysis --- p.66 Chapter 2.3 --- Results --- p.67 Chapter 2.3.1 --- Effects of CHL on the Growth of Human Cancer Cells by MTT Assay --- p.67 Chapter 2.3.2 --- Effect of CHL on the Proliferation of MCF-7 Cells by Chemi-BrdU Incorporation --- p.69 Chapter 2.3.3 --- Effect of CHL on Cell Cycle of MCF-7 Cells --- p.71 Chapter 2.3.4 --- Effect of CHL on the Cyclin D1 Expression in MCF-7 Cells --- p.74 Chapter 2.3.5 --- Effects of CHL on JNK and c-Jun Expressions and Their Phosphorylations in MCF-7 Cells --- p.76 Chapter 2.3.6 --- Effect of CHL on DNA fragmentation in MCF-7 Cells --- p.78 Chapter 2.3.7 --- Effect of CHL on Mitochondrial Membrane Potential of MCF-7 Cells --- p.80 Chapter 2.3.8 --- Effects of CHL on the PARP Expression and Cleavage in MCF-7 Cells --- p.83 Chapter 2.3.9 --- "Effects of CHL on Bcl-2, Bcl-xL and Bad Expressions in MCF-7 Cells" --- p.85 Chapter 2.3.10 --- Effects of CHL on Caspase Activations in MCF-7 Cells --- p.88 Chapter 2.3.11 --- Effects of Caspase Inhibitors on the CHL-Induced Apoptosis in MCF-7 Cells --- p.90 Chapter 2.4 --- Discussion --- p.93 Chapter Chapter 3 --- In Vivo Studies of the Anticancer Effect of Chlorophyllin Chapter 3.1 --- Introduction --- p.104 Chapter 3.2 --- Materials and Methods --- p.106 Chapter 3.2.1 --- Transplantation of MCF-7 Cells into the Nude Mice and Treatment --- p.106 Chapter 3.2.2 --- Western Blot Analysis --- p.107 Chapter 3.2.3 --- Statistical Analysis --- p.107 Chapter 3.3 --- Results --- p.108 Chapter 3.3.1 --- In Vivo Antitumor Activity of CHL --- p.108 Chapter 3.3.2 --- In Vivo Effects of CHL on Cyclin D1 and Bcl-2 Expressions in MCF-7 Solid Tumor --- p.111 Chapter 3.4 --- Discussion --- p.113 Chapter Chapter 4 --- General Discussion --- p.115 References --- p.126 Kong, Ka-lai. Chinese University of Hong Kong Graduate School. Division of Biology. 2005 Text bibliography print xvii, 149 leaves : ill. (some col.) ; 30 cm. cuhk:325348 http://library.cuhk.edu.hk/record=b5892491 eng chi Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) http://repository.lib.cuhk.edu.hk/en/islandora/object/cuhk%3A325348/datastream/TN/view/Growth%20inhibitory%20effects%20of%20chlorophyllin%20on%20human%20breast%20carcinoma%20MCF-7%20cells.jpghttp://repository.lib.cuhk.edu.hk/en/item/cuhk-325348 |