Migration of mouse sacral neural crest cells.

• Other Authors: Dong, Ming.
• Format: Others
• Language: English; Chinese
• Published: 2006
• Subjects: Neural crest; Cell migration; Mice > Embryology; Neural Crest; Cell Movement; Nervous System > embryology; Nervous System > growth & development; Mice > embryology
• Online Access: http://library.cuhk.edu.hk/record=b5892802; http://repository.lib.cuhk.edu.hk/en/item/cuhk-325497

Dong Ming. === Thesis submitted in: December 2005. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. === Includes bibliographical references (leaves 118-152). === Abstracts in English and Chinese. === Abstract (English) --- p.i === Abstract (Chinese) --- p.iii === Acknowledgement --- p.iv === Table of Contents --- p.v === Abbreviation list --- p.xi === Chapter Chapter 1 --- General introduction === Chapter 1.1 --- Preamble --- p.1 === Chapter 1.2 --- Neural Crest Cells (NCCs) --- p.2 === Chapter 1.3 --- Enteric Nervous System (ENS) and Vagal Neural Crest Cells (Vagal NCCs) --- p.4 === Chapter 1.4 --- Sacral Neural Crest Cells (Sacral NCCs) --- p.7 === Chapter 1.5 --- Signalling Mechanisms of Sacral Neural Crest Cells --- p.17 === Chapter 1.6 --- Hirschsprung's Disease (HSCR) --- p.20 === Chapter 1.7 --- Objective of the Study and Contents of the Following Chapters --- p.21 === Chapter Chapter 2 --- Migration from the dorsal neural tube to the pelvic mesenchyme === Chapter 2.1 --- Introduction --- p.24 === Chapter 2.2 --- Materials and Methods --- p.32 === Chapter 2.2.1 --- Animal --- p.32 === Chapter 2.2.2 --- Isolation of embryos from pregnant mice at E9.5 to --- p.32 === Chapter 2.2.3 --- Histological preparation of the caudal segments --- p.33 === Chapter 2.2.4 --- p75 immunohistochemical staining --- p.33 === Chapter 2.2.5 --- Preparation of rat serum --- p.34 === Chapter 2.2.6 --- Preparation of the culture medium --- p.34 === Chapter 2.2.7 --- Preparation of wheat germ agglutinin-gold conjugates (WGA-Au) --- p.35 === Chapter 2.2.8 --- Preparation of CMFDA --- p.35 === Chapter 2.2.9 --- Preparation of DiI --- p.36 === Chapter 2.2.10 --- "Microinjection of WGA-Au, DiI and CMFDA" --- p.36 === Chapter 2.2.11 --- Whole embryo culture --- p.37 === Chapter 2.2.12 --- Examination of cultured embryos --- p.37 === Chapter 2.2.13 --- Histological preparation of WGA-Au labelled embryos --- p.38 === Chapter 2.2.14 --- Silver enhancement staining of the sections of WGA-Au labelled embryos --- p.39 === Chapter 2.2.15 --- Cryosectioning of the embryos labelled with DiI --- p.39 === Chapter 2.2.16 --- p75 immunohistochemical staining of DiI-labelled cells --- p.40 === Chapter 2.3 --- Results --- p.41 === Chapter 2.3.1 --- Observations on embryos developed in vivo --- p.41 === Chapter 2.3.2 --- Closed yolk sac culture vs open yolk sac culture --- p.42 === Chapter 2.3.3 --- Neural crest cell labelling in the caudal part of embryos --- p.43 === Chapter 2.3.4 --- Neural crest cell labelling with DiI in the caudal part of the neural tube followed by in vitro culture from E9.5 to E11.0 --- p.45 === Chapter 2.3.5 --- Neural crest labelling with DiI in the caudal part of the neural tube followed by in vitro culture from E10.5 to E11.5 --- p.46 === Chapter 2.3.6 --- Focal labelling at the levels of the 26th and 29th somites followed by in vitro culture --- p.48 === Chapter 2.3.7 --- p75 immunohistochemical staining on the caudal part of the embryo at E10.5 --- p.49 === Chapter 2.3.8 --- p75 immunohistochemical staining on embryos labelled with DiI --- p.50 === Chapter 2.4 --- Discussion --- p.51 === Chapter 2.4.1 --- Embryos at E9.5 cultured with an intact yolk sac membrane grew better than those with the yolk sac membrane cut open --- p.52 === Chapter 2.4.2 --- Migration at the levels of the 24th to 28th somite --- p.53 === Chapter 2.4.3 --- Migration at the levels of the 29th to 33th somite --- p.58 === Chapter 2.4.4 --- Sacral NCCs migrate along a straight dorsolateral pathway --- p.60 === Chapter 2.4.5 --- "Most of the DiI positive cells are p75 positive, but not all of the p75 positive cells are DiI positive" --- p.62 === Chapter Chapter 3 --- Migration from the pelvic mesenchyme to the hindgut === Chapter 3.1 --- Introduction --- p.65 === Chapter 3.2 --- Materials and Methods --- p.73 === Chapter 3.2.1 --- Isolation of hindguts with or without adjacent tissues from embryos at E10.5 to E13.5 --- p.73 === Chapter 3.2.2 --- Microinjection of DiI into the pelvic mesenchymal tissue of the h indguts --- p.74 === Chapter 3.2.3 --- Preparation of the culture medium --- p.74 === Chapter 3.2.4 --- Preparation of the culture dish --- p.74 === Chapter 3.2.5 --- Gut culture --- p.75 === Chapter 3.2.6 --- Cryosections of the hindguts after in vitro culture --- p.75 === Chapter 3.3 --- Results --- p.76 === Chapter 3.3.1 --- "Hindguts isolated from embryos at E10.5, E11.5, E12.5 and E14.5" --- p.76 === Chapter 3.3.2 --- p75 immunohistochemical staining of the serial sections through the caudal part of the embryos --- p.78 === Chapter 3.3.3 --- Observations on hindgut without pelvic plexus cultured from E11.5 to E14.5 --- p.81 === Chapter 3.3.4 --- "Culture of hindguts with pelvic mesenchyme cultured from E11.5 to E14.25, E14.5 and E15.5" --- p.82 === Chapter 3.3.5 --- Culture of the whole length of the gut tube without pelvic mesenchyme from E11.5 to E15.5 --- p.84 === Chapter 3.3.6 --- Culture of the whole length of the gut tube with pelvic mesenchyme from E11.5 to E15.5 --- p.84 === Chapter 3.3.7 --- "Culture of hindguts with Dil labelling in the pelvic mesenchyme from E11.5 to E14.0, E14.2 5, E14.5 and" --- p.85 === Chapter 3.3.8 --- Culture of the whole length of the gut tube with DiI labelling in the pelvic mesenchyme from E11.5 to E14.5 --- p.86 === Chapter 3.4 --- Discussion --- p.88 === Chapter 3.4.1 --- Development of the hindgut and the urogenital system from E10.5 to E14.5 --- p.88 === Chapter 3.4.2 --- No p75 positive cells were found in the hindgut before E13.5 --- p.89 === Chapter 3.4.3 --- The sacral neural crest cells migrate into the hindgut at around E14.5 --- p.91 === Chapter 3.4.4 --- "The sacral neural crest cells migrated in the serosa, and entered the myenteric plexus prior to populating the submucosal plexus" --- p.95 === Chapter 3.4.5 --- Most of DiI labelled sacral neural crest cells in the hindgut also expressed p75 --- p.98 === Chapter Chapter 4 --- Migration from the neural tube to the hindgut === Chapter 4.1 --- Introduction --- p.101 === Chapter 4.2 --- Materials and Methods --- p.104 === Chapter 4.3 --- Results --- p.106 === Chapter 4.3.1 --- Morphology observations on the hindguts isolated from DiI-labelled embryos --- p.106 === Chapter 4.3.2 --- Distribution of the DiI-labelled cells and p75 positive cells before the culture of the hindgut explant --- p.106 === Chapter 4.3.3 --- Culture of hindgut explanted from Dil-labelled embyos for 3.5 days from E11.0 to E14.5 --- p.107 === Chapter 4.4 --- Discussion --- p.109 === Chapter Chapter 5 --- General discussion and conclusions --- p.113 === References --- p.118 === Figures and Legends --- p.153 === Tables and Graphs --- p.203 === Appendix --- p.209