Cellular consequence and molecular mechanism of reversal of apoptosis in mammalian cells.
Mak, Keng Hou. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. === Includes bibliographical references (leaves 77-91). === Abstracts in English and Chinese. === Thesis Committee --- p.2 === Declaration --- p.3 === Table of Contents --- p.4 === List of Abbreviations --- p.6 === List...
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| Format: | Others |
| Language: | English Chinese |
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2011
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| Online Access: | http://library.cuhk.edu.hk/record=b5894639 http://repository.lib.cuhk.edu.hk/en/item/cuhk-327396 |
| Summary: | Mak, Keng Hou. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. === Includes bibliographical references (leaves 77-91). === Abstracts in English and Chinese. === Thesis Committee --- p.2 === Declaration --- p.3 === Table of Contents --- p.4 === List of Abbreviations --- p.6 === List of Figures --- p.8 === Abstract --- p.10 === Chapter Chapter 1 --- Introduction --- p.12 === Chapter 1.1 --- Background --- p.12 === Chapter 1.1.1 --- Overview of apoptosis --- p.12 === Chapter 1.1.2 --- Synopsis of the apoptotic pathway --- p.13 === Chapter 1.1.3 --- Defining apoptosis --- p.14 === Chapter 1.1.4 --- Interaction between pro- and anti-apoptotic factors determines cell fate --- p.14 === Chapter 1.1.5 --- DNA fragmentation during the execution phase --- p.15 === Chapter 1.1.6 --- Current understanding of the point of commitment in apoptosis --- p.16 === Chapter 1.1.7 --- Previous studies and hypotheses related to the reversibility of late-state apoptosis --- p.16 === Chapter 1.1.8 --- Unanswered questions --- p.19 === Chapter 1.2 --- "Hypothesis and objectives, Study models and Significance" --- p.19 === Chapter 1.2.1 --- Hypothesis and objectives --- p.19 === Chapter 1.2.2 --- Study models --- p.20 === Chapter 1.2.3 --- Significance --- p.20 === Chapter Chapter 2 --- Materials and Methods --- p.22 === Chapter Chapter 3 --- Results --- p.30 === Chapter 3.1 --- Dying cells reversed execution stage of apoptosis after removal of apoptotic stimuli --- p.30 === Chapter 3.2 --- Dying cells reversed apoptosis after DNA damage --- p.37 === Chapter 3.3 --- Genetic alterations and transformation occurred after reversal of apoptosis --- p.43 === Chapter 3.4 --- Investigating molecular mechanism driving reversal of apoptosis --- p.50 === Chapter 3.4.1 --- Preparation and characterization of samples for microarray --- p.50 === Chapter 3.4.2 --- Gene ontology enrichment analysis of the expression profile during reversal of apoptosis --- p.52 === Chapter 3.4.3 --- Interfering stress response or anti-apoptotic factors during the reversal of apoptosis drove cells to terminal death --- p.56 === Chapter Chapter 4 --- Discussion --- p.62 === Chapter 4.1 --- "Reversal of apoptosis in ""normal cells"" was observed" --- p.62 === Chapter 4.2 --- Cells surviving apoptosis had their genomes damaged and altered --- p.63 === Chapter 4.3 --- Transformation occurred after reversal of apoptosis --- p.65 === Chapter 4.4 --- Investigating molecular mechanism driving reversal of apoptosis --- p.65 === Chapter 4.5 --- Summary --- p.68 === Chapter Chapter 5 --- Perspectives --- p.70 === Chapter 5.1 --- Could reversal of apoptosis be evolutionarily advantageous? --- p.70 === Chapter 5.2 --- "Reversal of apoptosis as an ""individualistic"" behavior against organismal integrity" --- p.71 === Chapter 5.3 --- Proposed studies --- p.72 === Chapter 5.3.1 --- Other apoptotic targets that may leave persistent effects --- p.72 === Chapter 5.3.2 --- Post- caspase activation regulation of apoptosis --- p.74 === Chapter 5.3.3 --- Identifying correlation between reversal of apoptosis and cancer --- p.74 === Chapter 5.3.4 --- Single cell methods and cell tracking system for further studies --- p.75 === Chapter 5.3.5 --- Notes on studying reversal of apoptosis in relation to phagocytosis --- p.76 === References --- p.77 |
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