Functional analysis of an apoplast-localized BURP-domain protein (GmRD22) from soybean.
BURP域家族是植物特有的一個蛋白家族,結構多樣,共同點是在羧基端都有一個保守的BURP域。迄今為止,關於BURP域家族成員的功能及細胞定位的研究非常有限。部分RD22-like的亞家族成員由於顯示出受非生物脅迫而誘導表達的特性,因此被認為功能可能與非生物脅迫回應相關。本研究對克隆到的一個受非生物脅迫誘導表達的大豆基因(GmRD22)進行了生物進化分析,并詳細分析了其在不同的大豆品種以及不同非生物脅迫下的表達模式,揭示了其表達豐度與大豆的抗非生物脅迫能力有關,並且使用不同的轉基因系統(細胞水準跟植物水準)揭示了其過量表達有助於減輕非生物脅迫對植物造成的影響。研究利用GFP融合蛋白追蹤技術和免疫...
Other Authors: | Wang, Hongmei |
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Format: | Others |
Language: | English Chinese |
Published: |
2012
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Subjects: | |
Online Access: | http://library.cuhk.edu.hk/record=b5549489 http://repository.lib.cuhk.edu.hk/en/item/cuhk-328070 |
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