Highwire's characterization and signaling roles in Drosophila central synapse formation
The assembly and maintenance of central synapses is a complex process, requiring myriad genes and their products. Highwire is a large gene containing a RING domain, characteristic of ubiquitin E3 ligases. Highwire has been shown to restrain axon growth and control synaptogenesis at a peripheral syna...
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ndltd-fau.edu-oai-fau.digital.flvc.org-fau_39042019-07-04T03:54:52Z Highwire's characterization and signaling roles in Drosophila central synapse formation Rowland, Kimberly Diane. Text Electronic Thesis or Dissertation Florida Atlantic University English xi, 93 p. : ill. (some col.) electronic The assembly and maintenance of central synapses is a complex process, requiring myriad genes and their products. Highwire is a large gene containing a RING domain, characteristic of ubiquitin E3 ligases. Highwire has been shown to restrain axon growth and control synaptogenesis at a peripheral synapse. Here I examine the roles of Highwire at a central synapse in the adult Drosophila Giant Fiber System (GFS). Highwire is indeed necessary for proper axonal growth as well as synaptic transmission in the GFS. Differences arise between the central synapse and the neuromuscular junction (NMJ), where highwire was initially characterized : expresion from the postsynaptic cell can rescue highwire synaptic defects, which is not seen at the NMJ. In addition, a MAP kinase signaling pathway regulated by highwire at the NMJ has differing roles at a central synapse. Wallenda MAPK can rescue not only the highwire anatomical phenotype but also the defects seen in transmission. Another distinction is seen here : loss of function basket and Dfos enhance the highwire anatomical phenotype while expression of dominant negative basket and Dfos suppress the highwire phenotype. As a result we have compared the signaling pathway in flies and worms and found that the NMJ in the two organisms use a parallel pathway while the central synapse uses a distinct pathway. by Kimberly Diane Rowland. Thesis (Ph.D.)--Florida Atlantic University, 2012. Includes bibliography. Electronic reproduction. Boca Raton, Fla., 2012. Mode of access: World Wide Web. Cellular control mechanisms Cellular signal transduction Cell differentiation Gene expression Genetic transcription Transcription factors Drosophila melanogaster--Cytogenetics http://purl.flvc.org/FAU/3352826 810269276 3352826 FADT3352826 fau:3904 Charles E. Schmidt College of Science Department of Biological Sciences http://rightsstatements.org/vocab/InC/1.0/ https://fau.digital.flvc.org/islandora/object/fau%3A3904/datastream/TN/view/Highwire%27s%20characterization%20and%20signaling%20roles%20in%20Drosophila%20central%20synapse%20formation.jpg |
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Cellular control mechanisms Cellular signal transduction Cell differentiation Gene expression Genetic transcription Transcription factors Drosophila melanogaster--Cytogenetics |
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Cellular control mechanisms Cellular signal transduction Cell differentiation Gene expression Genetic transcription Transcription factors Drosophila melanogaster--Cytogenetics Highwire's characterization and signaling roles in Drosophila central synapse formation |
description |
The assembly and maintenance of central synapses is a complex process, requiring myriad genes and their products. Highwire is a large gene containing a RING domain, characteristic of ubiquitin E3 ligases. Highwire has been shown to restrain axon growth and control synaptogenesis at a peripheral synapse. Here I examine the roles of Highwire at a central synapse in the adult Drosophila Giant Fiber System (GFS). Highwire is indeed necessary for proper axonal growth as well as synaptic transmission in the GFS. Differences arise between the central synapse and the neuromuscular junction (NMJ), where highwire was initially characterized : expresion from the postsynaptic cell can rescue highwire synaptic defects, which is not seen at the NMJ. In addition, a MAP kinase signaling pathway regulated by highwire at the NMJ has differing roles at a central synapse. Wallenda MAPK can rescue not only the highwire anatomical phenotype but also the defects seen in transmission. Another distinction is seen here : loss of function basket and Dfos enhance the highwire anatomical phenotype while expression of dominant negative basket and Dfos suppress the highwire phenotype. As a result we have compared the signaling pathway in flies and worms and found that the NMJ in the two organisms use a parallel pathway while the central synapse uses a distinct pathway. === by Kimberly Diane Rowland. === Thesis (Ph.D.)--Florida Atlantic University, 2012. === Includes bibliography. === Electronic reproduction. Boca Raton, Fla., 2012. Mode of access: World Wide Web. |
author2 |
Rowland, Kimberly Diane. |
author_facet |
Rowland, Kimberly Diane. |
title |
Highwire's characterization and signaling roles in Drosophila central synapse formation |
title_short |
Highwire's characterization and signaling roles in Drosophila central synapse formation |
title_full |
Highwire's characterization and signaling roles in Drosophila central synapse formation |
title_fullStr |
Highwire's characterization and signaling roles in Drosophila central synapse formation |
title_full_unstemmed |
Highwire's characterization and signaling roles in Drosophila central synapse formation |
title_sort |
highwire's characterization and signaling roles in drosophila central synapse formation |
publisher |
Florida Atlantic University |
url |
http://purl.flvc.org/FAU/3352826 |
_version_ |
1719219365547606016 |