Characterization of Oscillatoria spp. and their Role in Black Band Disease of Coral
Black band disease (BBD) is a cyanobacterial dominated pathogenic consortium that affects corals worldwide. Recently two cyanobacteria (Oscillatoria strains 101-1 and 100-1) were isolated into culture from BBD. The aim of this study was to characterize the strains and assess their role in BBD pathog...
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ndltd-fiu.edu-oai-digitalcommons.fiu.edu-etd-13072018-01-05T15:34:32Z Characterization of Oscillatoria spp. and their Role in Black Band Disease of Coral Stanic, Dina Black band disease (BBD) is a cyanobacterial dominated pathogenic consortium that affects corals worldwide. Recently two cyanobacteria (Oscillatoria strains 101-1 and 100-1) were isolated into culture from BBD. The aim of this study was to characterize the strains and assess their role in BBD pathogenesis. Light, scanning electron and transmission electron microscopy, coupled with 16S rRNA gene sequencing, were used for taxonomic characterization. Cyanotoxin production was assessed by enzyme-linked immunosorbent assay. Toxin identification was performed by high performance liquid chromatography. The ability of the strains to initiate BBD was tested on host coral fragments of Siderastrea siderea and Diploria strigosa under controlled laboratory conditions. Results showed that both Oscillatoria sp. strains caused infection that resulted in complete lysis of coral tissue. Both strains produced a cyanotoxin, identified as microcystin-LR, with production affected by different environmental factors. This study provides evidence that BBD Oscillatoria is a key component in BBD pathogenicity. 2010-07-12T07:00:00Z text application/pdf http://digitalcommons.fiu.edu/etd/243 http://digitalcommons.fiu.edu/cgi/viewcontent.cgi?article=1307&context=etd FIU Electronic Theses and Dissertations FIU Digital Commons Black band disease cyanobacteria microcystin Life Sciences |
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Black band disease cyanobacteria microcystin Life Sciences |
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Black band disease cyanobacteria microcystin Life Sciences Stanic, Dina Characterization of Oscillatoria spp. and their Role in Black Band Disease of Coral |
description |
Black band disease (BBD) is a cyanobacterial dominated pathogenic consortium that affects corals worldwide. Recently two cyanobacteria (Oscillatoria strains 101-1 and 100-1) were isolated into culture from BBD. The aim of this study was to characterize the strains and assess their role in BBD pathogenesis. Light, scanning electron and transmission electron microscopy, coupled with 16S rRNA gene sequencing, were used for taxonomic characterization. Cyanotoxin production was assessed by enzyme-linked immunosorbent assay. Toxin identification was performed by high performance liquid chromatography. The ability of the strains to initiate BBD was tested on host coral fragments of Siderastrea siderea and Diploria strigosa under controlled laboratory conditions. Results showed that both Oscillatoria sp. strains caused infection that resulted in complete lysis of coral tissue. Both strains produced a cyanotoxin, identified as microcystin-LR, with production affected by different environmental factors. This study provides evidence that BBD Oscillatoria is a key component in BBD pathogenicity. |
author |
Stanic, Dina |
author_facet |
Stanic, Dina |
author_sort |
Stanic, Dina |
title |
Characterization of Oscillatoria spp. and their Role in Black Band Disease of Coral |
title_short |
Characterization of Oscillatoria spp. and their Role in Black Band Disease of Coral |
title_full |
Characterization of Oscillatoria spp. and their Role in Black Band Disease of Coral |
title_fullStr |
Characterization of Oscillatoria spp. and their Role in Black Band Disease of Coral |
title_full_unstemmed |
Characterization of Oscillatoria spp. and their Role in Black Band Disease of Coral |
title_sort |
characterization of oscillatoria spp. and their role in black band disease of coral |
publisher |
FIU Digital Commons |
publishDate |
2010 |
url |
http://digitalcommons.fiu.edu/etd/243 http://digitalcommons.fiu.edu/cgi/viewcontent.cgi?article=1307&context=etd |
work_keys_str_mv |
AT stanicdina characterizationofoscillatoriasppandtheirroleinblackbanddiseaseofcoral |
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1718581384363114496 |