Nanomaterials: Synthesis, Characterization, and Their Bio-Integration
The purpose of this dissertation is to utilize the changing optical and electronic properties of metallic and semiconductor nanomaterials for applications to biotechnology. The dynamic optical properties of metals and semiconductors with size is discussed in regard to the ability of these materials...
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| Format: | Others |
| Language: | English English |
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Florida State University
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| Online Access: | http://purl.flvc.org/fsu/fd/FSU_migr_etd-3532 |
| Summary: | The purpose of this dissertation is to utilize the changing optical and electronic properties of metallic and semiconductor nanomaterials for applications to biotechnology. The dynamic optical properties of metals and semiconductors with size is discussed in regard to the ability of these materials to accept electronic excitation energy from classical molecular fluorescent dyes (Chap. 2). Absorption, photoluminescence, and time-resolved photoluminescence experiments are performed on metal nanoparticle-dye pairs at separation distances controlled via synthetic DNA spacers where the distance, dye, and nanoparticle size are varied (Chaps. 3 and 4). It is found that the efficiency of energy transfer to small metal nanoparticles is greater than expected for a 1/R^6 Förster mechanism of energy transfer and the measurable separation distance is increased, following a 1/R^4 dependence. The 1/R^4 distance dependence is the theoretically established relationship of an excited molecule to a metallic surface. This tool, termed "Nanometal Surface Energy Transfer" (NSET) is then used in Chapters 5 and 6 to measure the kinetics and conformational changes associated with a hammerhead ribozyme as a model test subject for NSET methodology. Finally, ZnS-overcoated CdSe semiconductor quantum dots, (Chap. 7) are synthesized controllably and characterized in terms of their potential for biological incorporation for detection or in vitro studies. === A Dissertation submitted to the Department of Chemistry and Biochemistry in partial fulfillment of the requirements for the degree of Doctor of
Philosophy. === Spring Semester, 2006. === January 18, 2006. === Nanoparticle, Energy Transfer, FRET, Quantum Dot, Nanomaterial === Includes bibliographical references. === Geoffrey Strouse, Professor Directing Dissertation; Bryant Chase, Outside Committee Member; Michael Kasha, Committee Member; Harry W. Kroto, Committee Member. |
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