Effect of smoking and waist circumference on biochemical markers of oxidative stress in subjects with IGT and newly diagnosed diabetics from Bellville South, Western Cape, South Africa

• Main Author: Tjaronda, Timothy Ngatangwe
• Other Authors: Matsha, T.
• Language: en
• Published: Cape Peninsula University of Technology 2016
• Subjects: Oxidative stress; Diabetes mellitus; Obesity; Smoking; Atherosclerosis; Impaired glucose tolerance
• Online Access: http://hdl.handle.net/20.500.11838/2241

Thesis (MTech (Biomedical Technology))--Cape Peninsula University of Technology, 2011. === Studies have shown that oxidative stress (OS) is a major pathological risk factor in various diseases, including type 2 diabetes mellitus (T2DM). Hyperglycemia independently is a generator of free radicals, hence increases the level of OS in T2DM subjects. The oxidation of LDL is suggested to play a significant role in the pathogenesis of macrovascular complications observed in diabetic patients. In subjects with hyperglycemia or normoglycemia we investigated the relationship between MDA-protein adducts, HNE-protein adducts and auto-antibodies against oxLDL, and cardiovascular profile as measured by hs- CRP. From an epidemiological study that screened a high risk urban population for diabetes using oral glucose tolerance test, 98 hyperglycaemie and 79 normoglycaemic individuals were selected for this study. Enzyme linked immuno-sorbent-assay methods were used to determine the levels of serum MDA-protein adducts, HNE-protein adducts or auto-antibodies against oxLDL. High sensitive CRP was measured by nephelometry.