Analysis of genes and enzymes involved in the degradation of hemicellulose and cellulose by Butyrivibrio fibrisolvens H17c

Bibliography: pages 182-223. === B. fibrisolvens H17c is a Gram-positive obligate anaerobe which has been found in the rumen of most ruminants. Strains of B. fibrisol vens have been reported to exhibit activity toward cellulosic and hemicellulosic substrates. The aim of this thesis was to screen a g...

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Bibliographic Details
Main Author: Lin, Long-Liu
Other Authors: Thomson, Jennifer Ann
Format: Doctoral Thesis
Language:English
Published: University of Cape Town 2016
Subjects:
Online Access:http://hdl.handle.net/11427/21825
Description
Summary:Bibliography: pages 182-223. === B. fibrisolvens H17c is a Gram-positive obligate anaerobe which has been found in the rumen of most ruminants. Strains of B. fibrisol vens have been reported to exhibit activity toward cellulosic and hemicellulosic substrates. The aim of this thesis was to screen a genebank of B. fibrisolvens H17c DNA and to isolate genes expressing cellulase and xylanase activity. Two genes encoding β-1-4- glucosidase (BglA) and endo-β-1-4-xylanase (XynB) were cloned in E. coli.