The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction

Gonadotropin-releasing hormone (GnRH), a central regulator of reproductive function in all vertebrates, exerts its effects via binding to the GnRH receptor (GnRHR) in the pituitary gonadotrophs. The GnRHR is a member of the G-protein coupled receptor (GPCR) superfarnily. A second form of the GnRHR (...

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Main Author: Hutchinson, Emerentia
Format: Dissertation
Language:English
Published: University of Cape Town 2018
Subjects:
Online Access:http://hdl.handle.net/11427/26968
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spelling ndltd-netd.ac.za-oai-union.ndltd.org-uct-oai-localhost-11427-269682020-10-06T05:11:22Z The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction Hutchinson, Emerentia Chemical Pathology Gonadotropin-releasing hormone (GnRH), a central regulator of reproductive function in all vertebrates, exerts its effects via binding to the GnRH receptor (GnRHR) in the pituitary gonadotrophs. The GnRHR is a member of the G-protein coupled receptor (GPCR) superfarnily. A second form of the GnRHR (type II), other than the pituitary gonadotrope GnRHR (type I) has been proposed to exist and to play a role other than the classical endocrine role of the pituitary GnRHR. Elucidation of amino acid residues of the GnRHR that are crucial for ligand binding, activation of the receptor, and coupling to the G-protein, is important in understanding structure-function relationships towards the design of drugs for therapeutic intervention. Such information can often be deduced by a comparison between conserved and non-conserved amino acid residues of GnRHRs from different species. At the start of this project no non-mammalian or invertebrate, and only some of the eutherian mammalian type I GnRHRs had been cloned. The aim of this project was to clone novel GnRHRs, i.e. type I and type II GnRHRs from redbait and mole and type II mouse and human GnRHRs using polymerase chain reaction (PCR) strategies. PCR was performed with degenerate primers designed to human type I GnRHR to areas that are not conserved between GPCRs in general, but are conserved between mammalian GnRHRs. 2018-01-25T13:53:58Z 2018-01-25T13:53:58Z 1998 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/26968 eng application/pdf University of Cape Town Faculty of Health Sciences Division of Chemical Pathology
collection NDLTD
language English
format Dissertation
sources NDLTD
topic Chemical Pathology
spellingShingle Chemical Pathology
Hutchinson, Emerentia
The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction
description Gonadotropin-releasing hormone (GnRH), a central regulator of reproductive function in all vertebrates, exerts its effects via binding to the GnRH receptor (GnRHR) in the pituitary gonadotrophs. The GnRHR is a member of the G-protein coupled receptor (GPCR) superfarnily. A second form of the GnRHR (type II), other than the pituitary gonadotrope GnRHR (type I) has been proposed to exist and to play a role other than the classical endocrine role of the pituitary GnRHR. Elucidation of amino acid residues of the GnRHR that are crucial for ligand binding, activation of the receptor, and coupling to the G-protein, is important in understanding structure-function relationships towards the design of drugs for therapeutic intervention. Such information can often be deduced by a comparison between conserved and non-conserved amino acid residues of GnRHRs from different species. At the start of this project no non-mammalian or invertebrate, and only some of the eutherian mammalian type I GnRHRs had been cloned. The aim of this project was to clone novel GnRHRs, i.e. type I and type II GnRHRs from redbait and mole and type II mouse and human GnRHRs using polymerase chain reaction (PCR) strategies. PCR was performed with degenerate primers designed to human type I GnRHR to areas that are not conserved between GPCRs in general, but are conserved between mammalian GnRHRs.
author Hutchinson, Emerentia
author_facet Hutchinson, Emerentia
author_sort Hutchinson, Emerentia
title The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction
title_short The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction
title_full The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction
title_fullStr The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction
title_full_unstemmed The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction
title_sort cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction
publisher University of Cape Town
publishDate 2018
url http://hdl.handle.net/11427/26968
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