Characterisation of the human α2(I) procollagen promoter-binding proteins
In an attempt to elucidate the transcriptional mechanisms that regulate the expression of the human α2(I) procollagen gene, cis-acting DNA-elements within the proximal promoter were identified and their corresponding trans-acting factors characterised. The fibroblast cell lines used in this study ha...
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University of Cape Town
2018
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Online Access: | http://hdl.handle.net/11427/27139 |
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ndltd-netd.ac.za-oai-union.ndltd.org-uct-oai-localhost-11427-271392020-12-10T05:11:17Z Characterisation of the human α2(I) procollagen promoter-binding proteins Collins, Malcolm Robert Parker, M Iqbal DNA-Binding Proteins - analysis Procollagen - analysis Protein binding In an attempt to elucidate the transcriptional mechanisms that regulate the expression of the human α2(I) procollagen gene, cis-acting DNA-elements within the proximal promoter were identified and their corresponding trans-acting factors characterised. The fibroblast cell lines used in this study had previously been transformed with either simian virus 40 (SVWI-38) or by γ-radiation (CT-1). The SVWI-38 fibroblasts do not produce any α2(I) collagen chains, whereas the CT-1 cell line produces normal type I collagen. Previous studies suggested that trans-acting factor(s) may be responsible for the inactivation of the α2(I) procollagen gene in SVWI-38 fibroblasts (Parker et. al. (1989) J. Biol. Chem 264, 7147-7152; Parker et. al. (1992) Nucleic Acids Res. 20, 5825-5830). In this study, the SVWI-38 proximal promoter (-350 to +54) was sequenced and shown to be normal, thereby ruling out any possibility that mutations within this region was responsible for inactivation of the gene. 2018-01-30T14:00:25Z 2018-01-30T14:00:25Z 1993 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/27139 eng application/pdf University of Cape Town Faculty of Health Sciences Division of Medical Biochemistry and Structural Biology |
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English |
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Doctoral Thesis |
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DNA-Binding Proteins - analysis Procollagen - analysis Protein binding |
spellingShingle |
DNA-Binding Proteins - analysis Procollagen - analysis Protein binding Collins, Malcolm Robert Characterisation of the human α2(I) procollagen promoter-binding proteins |
description |
In an attempt to elucidate the transcriptional mechanisms that regulate the expression of the human α2(I) procollagen gene, cis-acting DNA-elements within the proximal promoter were identified and their corresponding trans-acting factors characterised. The fibroblast cell lines used in this study had previously been transformed with either simian virus 40 (SVWI-38) or by γ-radiation (CT-1). The SVWI-38 fibroblasts do not produce any α2(I) collagen chains, whereas the CT-1 cell line produces normal type I collagen. Previous studies suggested that trans-acting factor(s) may be responsible for the inactivation of the α2(I) procollagen gene in SVWI-38 fibroblasts (Parker et. al. (1989) J. Biol. Chem 264, 7147-7152; Parker et. al. (1992) Nucleic Acids Res. 20, 5825-5830). In this study, the SVWI-38 proximal promoter (-350 to +54) was sequenced and shown to be normal, thereby ruling out any possibility that mutations within this region was responsible for inactivation of the gene. |
author2 |
Parker, M Iqbal |
author_facet |
Parker, M Iqbal Collins, Malcolm Robert |
author |
Collins, Malcolm Robert |
author_sort |
Collins, Malcolm Robert |
title |
Characterisation of the human α2(I) procollagen promoter-binding proteins |
title_short |
Characterisation of the human α2(I) procollagen promoter-binding proteins |
title_full |
Characterisation of the human α2(I) procollagen promoter-binding proteins |
title_fullStr |
Characterisation of the human α2(I) procollagen promoter-binding proteins |
title_full_unstemmed |
Characterisation of the human α2(I) procollagen promoter-binding proteins |
title_sort |
characterisation of the human α2(i) procollagen promoter-binding proteins |
publisher |
University of Cape Town |
publishDate |
2018 |
url |
http://hdl.handle.net/11427/27139 |
work_keys_str_mv |
AT collinsmalcolmrobert characterisationofthehumana2iprocollagenpromoterbindingproteins |
_version_ |
1719370208092618752 |