Analysis of the role of nuclear factor-kappa B in insulin resistance caused by antiretroviral drugs

• Main Author: Mabugana, Matamela Charles
• Other Authors: Pillay, Tahir
• Language: en
• Published: University of Pretoria 2021
• Subjects: UCTD; Insulin resistance; HAART; CRISPR-Cas9; NFkB; Diabetes
• Online Access: http://hdl.handle.net/2263/79321; Mabugana, MC 2020, Analysis of the role of nuclear factor-kappa B in insulin resistance caused by antiretroviral drugs, MSc (Chemical Pathology) Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/79321>

Human immunodeficiency virus still remains the leading cause of death globally including women of child-bearing age. The rate of AIDS-related death has significantly declined since the introduction of antiretroviral treatment and other non-medical interventions such as the distribution and use of condoms. The introduction of antiretroviral treatment has however led to insulin resistance amongst users. Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated nuclease 9 (Cas) has been used to knockout NFκB to understand the pathway at which antiretroviral treatment causes insulin resistance. Heteroduplex mobility assay has shown that CRISPR-Cas9 knock out the gene of interest. These results have played a foundation in understanding how CRISPR-Cas9 can be integrated and utilized in medical research. === Dissertation (MSc (Chemical Pathology))--University of Pretoria, 2020. === National Research Foundation (NRF) === Chemical Pathology === MSc (Chemical Pathology) === Restricted