Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase

Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase

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Magister Scientiae - MSc === In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction chromatography. Amidase coupled enzyme stop assays were c...

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Bibliographic Details
Main Author: Mketsu, Moses Clive Masisange
Other Authors: Cowan, Don A.
Language:en
Published: University of the Western Cape 2014
Subjects:
Nitrogen compounds
Nitriles
Nitrogen
Online Access:http://hdl.handle.net/11394/2609
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spelling ndltd-netd.ac.za-oai-union.ndltd.org-uwc-oai-etd.uwc.ac.za-11394-26092017-08-02T04:00:16Z Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase Mketsu, Moses Clive Masisange Cowan, Don A. Tuffin, Marla van Zyl, Lonnie Dept. of Biotechnology Faculty of Science Nitrogen compounds Nitriles Nitrogen Magister Scientiae - MSc In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction chromatography. Amidase coupled enzyme stop assays were conducted using 3-cyanopyridine as a substrate, whereas continuous enzyme kinetics were conducted using acrylonitrile as a substrate. South Africa 2014-01-17T12:49:05Z 2011/06/08 08:31 2011/06/08 2014-01-17T12:49:05Z 2009 Thesis http://hdl.handle.net/11394/2609 en University of the Western Cape University of the Western Cape
collection NDLTD
language en
sources NDLTD
topic Nitrogen compounds
Nitriles
Nitrogen
spellingShingle Nitrogen compounds
Nitriles
Nitrogen
Mketsu, Moses Clive Masisange
Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
description Magister Scientiae - MSc === In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction chromatography. Amidase coupled enzyme stop assays were conducted using 3-cyanopyridine as a substrate, whereas continuous enzyme kinetics were conducted using acrylonitrile as a substrate. === South Africa
author2 Cowan, Don A.
author_facet Cowan, Don A.
Mketsu, Moses Clive Masisange
author Mketsu, Moses Clive Masisange
author_sort Mketsu, Moses Clive Masisange
title Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
title_short Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
title_full Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
title_fullStr Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
title_full_unstemmed Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
title_sort screening for subtate tolerant geobacillus pallidus rapc8 nitrile hydratase
publisher University of the Western Cape
publishDate 2014
url http://hdl.handle.net/11394/2609
work_keys_str_mv AT mketsumosesclivemasisange screeningforsubtatetolerantgeobacilluspallidusrapc8nitrilehydratase
_version_ 1718510415170764800

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