Investigation of the role of the ubiquitin-like DWNN domain in targeting Retinoblastoma Binding Protein 6 to nuclear speckles
Retinoblastoma Binding Protein 6 (RBBP6) is a 200 KDa protein shown to play a role in 3'- polyadenylation of mRNA transcripts, as well as to function as an E3 ligase catalysing ubiquitination of cancer-associated proteins. RBBP6 has been previously reported to localise to nuclear speckles, w...
Main Author: | |
---|---|
Other Authors: | |
Language: | en |
Published: |
University of the Western Cape
2018
|
Subjects: | |
Online Access: | http://hdl.handle.net/11394/6200 |
id |
ndltd-netd.ac.za-oai-union.ndltd.org-uwc-oai-etd.uwc.ac.za-11394-6200 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-netd.ac.za-oai-union.ndltd.org-uwc-oai-etd.uwc.ac.za-11394-62002018-08-08T04:42:14Z Investigation of the role of the ubiquitin-like DWNN domain in targeting Retinoblastoma Binding Protein 6 to nuclear speckles Mlaza, Mihlali Pugh, David J.R. RBBP6 DWNN domain Protein localisation Site-directed mutagenesis Nuclear speckles Retinoblastoma Binding Protein 6 (RBBP6) is a 200 KDa protein shown to play a role in 3'- polyadenylation of mRNA transcripts, as well as to function as an E3 ligase catalysing ubiquitination of cancer-associated proteins. RBBP6 has been previously reported to localise to nuclear speckles, which are thought to play a role in mRNA splicing, presumably as a result of its RS domain, which is known to target mRNA splicing factors to nuclear speckles. However recent studies in our laboratory have shown that isoform 3 of RBBP6, consisting mainly of the DWNN domain, also localises to speckles in resting cells, but more strongly in cells subjected to various stresses, suggesting that the DWNN domain may be the speckle-targeting domain. Magister Scientiae - MSc (Biotechnology) 2018-08-06T12:29:23Z 2018-08-06T12:29:23Z 2018 http://hdl.handle.net/11394/6200 en University of the Western Cape University of the Western Cape |
collection |
NDLTD |
language |
en |
sources |
NDLTD |
topic |
RBBP6 DWNN domain Protein localisation Site-directed mutagenesis Nuclear speckles |
spellingShingle |
RBBP6 DWNN domain Protein localisation Site-directed mutagenesis Nuclear speckles Mlaza, Mihlali Investigation of the role of the ubiquitin-like DWNN domain in targeting Retinoblastoma Binding Protein 6 to nuclear speckles |
description |
Retinoblastoma Binding Protein 6 (RBBP6) is a 200 KDa protein shown to play a role in 3'-
polyadenylation of mRNA transcripts, as well as to function as an E3 ligase catalysing
ubiquitination of cancer-associated proteins. RBBP6 has been previously reported to localise to
nuclear speckles, which are thought to play a role in mRNA splicing, presumably as a result of
its RS domain, which is known to target mRNA splicing factors to nuclear speckles. However
recent studies in our laboratory have shown that isoform 3 of RBBP6, consisting mainly of the
DWNN domain, also localises to speckles in resting cells, but more strongly in cells subjected to
various stresses, suggesting that the DWNN domain may be the speckle-targeting domain. === Magister Scientiae - MSc (Biotechnology) |
author2 |
Pugh, David J.R. |
author_facet |
Pugh, David J.R. Mlaza, Mihlali |
author |
Mlaza, Mihlali |
author_sort |
Mlaza, Mihlali |
title |
Investigation of the role of the ubiquitin-like DWNN domain in
targeting Retinoblastoma Binding Protein 6 to nuclear speckles |
title_short |
Investigation of the role of the ubiquitin-like DWNN domain in
targeting Retinoblastoma Binding Protein 6 to nuclear speckles |
title_full |
Investigation of the role of the ubiquitin-like DWNN domain in
targeting Retinoblastoma Binding Protein 6 to nuclear speckles |
title_fullStr |
Investigation of the role of the ubiquitin-like DWNN domain in
targeting Retinoblastoma Binding Protein 6 to nuclear speckles |
title_full_unstemmed |
Investigation of the role of the ubiquitin-like DWNN domain in
targeting Retinoblastoma Binding Protein 6 to nuclear speckles |
title_sort |
investigation of the role of the ubiquitin-like dwnn domain in
targeting retinoblastoma binding protein 6 to nuclear speckles |
publisher |
University of the Western Cape |
publishDate |
2018 |
url |
http://hdl.handle.net/11394/6200 |
work_keys_str_mv |
AT mlazamihlali investigationoftheroleoftheubiquitinlikedwnndomainintargetingretinoblastomabindingprotein6tonuclearspeckles |
_version_ |
1718724432519757824 |