Účinky oxidu uhličitého na neurovaskulární spojení během epileptické kortikální aktivity u potkanů
Aim: The aim of the study is to investigate the effect of CO 2 on neurovascular coupling in response to epileptiform activity after trancallosal electric stimulation in rats. Methods: Adult albino rats (250-350g, n=6) were anaesthetized with isoflurane and epidural silver EEG electrodes were implant...
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Online Access: | http://www.nusl.cz/ntk/nusl-276321 |
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ndltd-nusl.cz-oai-invenio.nusl.cz-2763212017-06-27T04:39:52Z Účinky oxidu uhličitého na neurovaskulární spojení během epileptické kortikální aktivity u potkanů Effect of carbon dioxide on neurovascular coupling during cortical epileptic activity in rats. Jindrová, Martina Otáhal, Jakub Brožíčková, Carole Aim: The aim of the study is to investigate the effect of CO 2 on neurovascular coupling in response to epileptiform activity after trancallosal electric stimulation in rats. Methods: Adult albino rats (250-350g, n=6) were anaesthetized with isoflurane and epidural silver EEG electrodes were implanted and fixed into the skull over sensorimotor cortices. To measure regional cerebral blood flow (rCBF) during epileptic activity a self-made metal holder for Laser Doppler Flowmeter (LDF) probe was fixed to thinned skull with dental acrylic. To measure arterial blood pressure (BP) and arterial blood gasses a plastic catheter was implanted into the common carotid artery. After postsurgical recovery animals were placed in a recording chamber. After 20 minutes of background recording effect of CO 2 on basal BP an rCBF was tested by inflating a mixture of 10%CO 2 , 20%O 2 and 70%N 2 for 30seconds. To assess changes in neurovascular coupling a rat model for myoclonic seizures was used. Biphasic constant current suprathreshold stimulus (8Hz, 15s) was applied under normal or elevated CO 2 atmosphere. Results: Elevated CO2 led to a significant increase of both regional cerebral blood flow and blood pressure. In addition, inhalation of 10%CO2 during electrical stimulation of senzorimotor cortex (8Hz, 15s) caused... 2010 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-276321 eng info:eu-repo/semantics/restrictedAccess |
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English |
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Dissertation |
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description |
Aim: The aim of the study is to investigate the effect of CO 2 on neurovascular coupling in response to epileptiform activity after trancallosal electric stimulation in rats. Methods: Adult albino rats (250-350g, n=6) were anaesthetized with isoflurane and epidural silver EEG electrodes were implanted and fixed into the skull over sensorimotor cortices. To measure regional cerebral blood flow (rCBF) during epileptic activity a self-made metal holder for Laser Doppler Flowmeter (LDF) probe was fixed to thinned skull with dental acrylic. To measure arterial blood pressure (BP) and arterial blood gasses a plastic catheter was implanted into the common carotid artery. After postsurgical recovery animals were placed in a recording chamber. After 20 minutes of background recording effect of CO 2 on basal BP an rCBF was tested by inflating a mixture of 10%CO 2 , 20%O 2 and 70%N 2 for 30seconds. To assess changes in neurovascular coupling a rat model for myoclonic seizures was used. Biphasic constant current suprathreshold stimulus (8Hz, 15s) was applied under normal or elevated CO 2 atmosphere. Results: Elevated CO2 led to a significant increase of both regional cerebral blood flow and blood pressure. In addition, inhalation of 10%CO2 during electrical stimulation of senzorimotor cortex (8Hz, 15s) caused... |
author2 |
Otáhal, Jakub |
author_facet |
Otáhal, Jakub Jindrová, Martina |
author |
Jindrová, Martina |
spellingShingle |
Jindrová, Martina Účinky oxidu uhličitého na neurovaskulární spojení během epileptické kortikální aktivity u potkanů |
author_sort |
Jindrová, Martina |
title |
Účinky oxidu uhličitého na neurovaskulární spojení během epileptické kortikální aktivity u potkanů |
title_short |
Účinky oxidu uhličitého na neurovaskulární spojení během epileptické kortikální aktivity u potkanů |
title_full |
Účinky oxidu uhličitého na neurovaskulární spojení během epileptické kortikální aktivity u potkanů |
title_fullStr |
Účinky oxidu uhličitého na neurovaskulární spojení během epileptické kortikální aktivity u potkanů |
title_full_unstemmed |
Účinky oxidu uhličitého na neurovaskulární spojení během epileptické kortikální aktivity u potkanů |
title_sort |
účinky oxidu uhličitého na neurovaskulární spojení během epileptické kortikální aktivity u potkanů |
publishDate |
2010 |
url |
http://www.nusl.cz/ntk/nusl-276321 |
work_keys_str_mv |
AT jindrovamartina ucinkyoxiduuhlicitehonaneurovaskularnispojenibehemepileptickekortikalniaktivityupotkanu AT jindrovamartina effectofcarbondioxideonneurovascularcouplingduringcorticalepilepticactivityinrats |
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