Adjuvantní slizniční imunizace myší proti chřipkovému viru

Influenza virus infections cause considerable morbidity and mortality in the world. Current immunization against influenza is provided using parenterally given influenza vaccines. These vaccines can induce good systemic immunity but they fail to induce a protective mucosal immunity. Because of persi...

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Main Author: Zanvit, Peter
Other Authors: Prokešová, Ludmila
Format: Doctoral Thesis
Language:English
Published: 2009
Online Access:http://www.nusl.cz/ntk/nusl-278730
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spelling ndltd-nusl.cz-oai-invenio.nusl.cz-2787302018-10-03T04:29:21Z Adjuvantní slizniční imunizace myší proti chřipkovému viru Adjuvant Mucosal Immunization of Mice against inFluenza Virus Zanvit, Peter Prokešová, Ludmila Tlaskalová - Hogenová, Helena Kopecký, Jan Influenza virus infections cause considerable morbidity and mortality in the world. Current immunization against influenza is provided using parenterally given influenza vaccines. These vaccines can induce good systemic immunity but they fail to induce a protective mucosal immunity. Because of persisting threat of new highly pathogenic influenza A subtypes development, a vaccination inducing intersubtypic cross-protection is desirable. The principal aims of the present study were: firstly, to explore the effect of Bacillus firmus (BF) and its delipidated form (DBF) as mucosal adjuvants for immunization via respiratory tract, secondly to test the potencial of BF and DBF to induce intrasubtypic and intersubtypic protection and finally to characterize the mechanism of adjuvant effect. (...) The mechanism of adjuvant effect was tested in NALT after intranasal immunization of mice by inactivated influenza virus type A, adjuvant alone (DBF) and by mixture of virus+DBF. We tested the expression of selected genes for cytokines, toll-like receptors and other genes participating in immune response by qPCR. Intranasally given DBF and mainly mixture virus+DBF induced expression of cytokines characteristic for Th1 immune response (IFN-and IL-2) whereas expression of genes characteristic for Th2 was decreased (IL-4).... 2009 info:eu-repo/semantics/doctoralThesis http://www.nusl.cz/ntk/nusl-278730 eng info:eu-repo/semantics/restrictedAccess
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language English
format Doctoral Thesis
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description Influenza virus infections cause considerable morbidity and mortality in the world. Current immunization against influenza is provided using parenterally given influenza vaccines. These vaccines can induce good systemic immunity but they fail to induce a protective mucosal immunity. Because of persisting threat of new highly pathogenic influenza A subtypes development, a vaccination inducing intersubtypic cross-protection is desirable. The principal aims of the present study were: firstly, to explore the effect of Bacillus firmus (BF) and its delipidated form (DBF) as mucosal adjuvants for immunization via respiratory tract, secondly to test the potencial of BF and DBF to induce intrasubtypic and intersubtypic protection and finally to characterize the mechanism of adjuvant effect. (...) The mechanism of adjuvant effect was tested in NALT after intranasal immunization of mice by inactivated influenza virus type A, adjuvant alone (DBF) and by mixture of virus+DBF. We tested the expression of selected genes for cytokines, toll-like receptors and other genes participating in immune response by qPCR. Intranasally given DBF and mainly mixture virus+DBF induced expression of cytokines characteristic for Th1 immune response (IFN-and IL-2) whereas expression of genes characteristic for Th2 was decreased (IL-4)....
author2 Prokešová, Ludmila
author_facet Prokešová, Ludmila
Zanvit, Peter
author Zanvit, Peter
spellingShingle Zanvit, Peter
Adjuvantní slizniční imunizace myší proti chřipkovému viru
author_sort Zanvit, Peter
title Adjuvantní slizniční imunizace myší proti chřipkovému viru
title_short Adjuvantní slizniční imunizace myší proti chřipkovému viru
title_full Adjuvantní slizniční imunizace myší proti chřipkovému viru
title_fullStr Adjuvantní slizniční imunizace myší proti chřipkovému viru
title_full_unstemmed Adjuvantní slizniční imunizace myší proti chřipkovému viru
title_sort adjuvantní slizniční imunizace myší proti chřipkovému viru
publishDate 2009
url http://www.nusl.cz/ntk/nusl-278730
work_keys_str_mv AT zanvitpeter adjuvantnisliznicniimunizacemysiprotichripkovemuviru
AT zanvitpeter adjuvantmucosalimmunizationofmiceagainstinfluenzavirus
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