HPLC analýza léčiv a potenciálních léčiv v biologickém materiálu
High-performance liquid chromatography (HPLC) is currently one of the most progressive separation methods, permitting, simultaneously, both quantitative and qualitative analysis. An important area for the utilisation of HPLC is the identification of drugs and their metabolites in biological material...
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2009
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Online Access: | http://www.nusl.cz/ntk/nusl-278922 |
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ndltd-nusl.cz-oai-invenio.nusl.cz-2789222017-06-27T04:40:30Z HPLC analýza léčiv a potenciálních léčiv v biologickém materiálu HPLC analysis of drugs and drug candidates in biological material Luňáková, Veronika Kovaříková, Petra Mokrý, Milan High-performance liquid chromatography (HPLC) is currently one of the most progressive separation methods, permitting, simultaneously, both quantitative and qualitative analysis. An important area for the utilisation of HPLC is the identification of drugs and their metabolites in biological materials. This study was focused on the development of chromatographic conditions appropriate for HPLC analysis of two new potential drugs derived from aroyl-hydrazone, HNAF-INH and 4,6DAR-INH, in biological material and its subsequent application in the assessment of the stability of these substances in plasma. Analysed chelators were prepared by the modification of the structure of salicylaldehyde isonicotinoyl hydrazone (SIH) with the purpose of improving the stability of the substance in plasma. As a suitable stationary phase the HPLC column LiChroCART® HPLC - cartridge LiChrospher®100 RP - 18e (15μm) with the pre-column LiChroCART® 4-4 Purospher® RP - 18 (5μm) was chosen. The mobile phase in this case represented by a HNAF-INH compound: phosphate buffer (0,01 mol/l aqueous solution of NaH2PO4 · 2 H2O with 2 mmol/l EDTA, pH 3) : methanol, 42:58 (v/v). The temperature in the column was 25řC, the flow rate 1,0 ml/min and UV detection was performed at 288 nm; SIH was used as an internal standard. The mobile... 2009 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-278922 cze info:eu-repo/semantics/restrictedAccess |
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Czech |
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Dissertation |
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description |
High-performance liquid chromatography (HPLC) is currently one of the most progressive separation methods, permitting, simultaneously, both quantitative and qualitative analysis. An important area for the utilisation of HPLC is the identification of drugs and their metabolites in biological materials. This study was focused on the development of chromatographic conditions appropriate for HPLC analysis of two new potential drugs derived from aroyl-hydrazone, HNAF-INH and 4,6DAR-INH, in biological material and its subsequent application in the assessment of the stability of these substances in plasma. Analysed chelators were prepared by the modification of the structure of salicylaldehyde isonicotinoyl hydrazone (SIH) with the purpose of improving the stability of the substance in plasma. As a suitable stationary phase the HPLC column LiChroCART® HPLC - cartridge LiChrospher®100 RP - 18e (15μm) with the pre-column LiChroCART® 4-4 Purospher® RP - 18 (5μm) was chosen. The mobile phase in this case represented by a HNAF-INH compound: phosphate buffer (0,01 mol/l aqueous solution of NaH2PO4 · 2 H2O with 2 mmol/l EDTA, pH 3) : methanol, 42:58 (v/v). The temperature in the column was 25řC, the flow rate 1,0 ml/min and UV detection was performed at 288 nm; SIH was used as an internal standard. The mobile... |
author2 |
Kovaříková, Petra |
author_facet |
Kovaříková, Petra Luňáková, Veronika |
author |
Luňáková, Veronika |
spellingShingle |
Luňáková, Veronika HPLC analýza léčiv a potenciálních léčiv v biologickém materiálu |
author_sort |
Luňáková, Veronika |
title |
HPLC analýza léčiv a potenciálních léčiv v biologickém materiálu |
title_short |
HPLC analýza léčiv a potenciálních léčiv v biologickém materiálu |
title_full |
HPLC analýza léčiv a potenciálních léčiv v biologickém materiálu |
title_fullStr |
HPLC analýza léčiv a potenciálních léčiv v biologickém materiálu |
title_full_unstemmed |
HPLC analýza léčiv a potenciálních léčiv v biologickém materiálu |
title_sort |
hplc analýza léčiv a potenciálních léčiv v biologickém materiálu |
publishDate |
2009 |
url |
http://www.nusl.cz/ntk/nusl-278922 |
work_keys_str_mv |
AT lunakovaveronika hplcanalyzalecivapotencialnichlecivvbiologickemmaterialu AT lunakovaveronika hplcanalysisofdrugsanddrugcandidatesinbiologicalmaterial |
_version_ |
1718468605507534848 |