Detekce protilátek reagujících s F. tularensis v časné fázi infekce

• Main Author: Straková, Kristina
• Other Authors: Janovská, Sylva
• Format: Dissertation
• Language: Czech
• Published: 2010
• Online Access: http://www.nusl.cz/ntk/nusl-279451

Kristina Straková Detection of antibodies reactive with F. tularensis in early phase of infection Thesis Charles University in Prague, Faculty Of Pharmacy in Hradec Králové Study program Pharmacy Because of its high virulence and mortality Francisella tularensis is considered to be a dangerous potential biological weapon and therefore this etiological agent is a suitable object of the primary research. Problems of an antibody response to the infection with intracellular pathogens are currently gaining in importance. Opinions and conclusions of various research teams differ, so this topic is up-to-date. The aim of this study was to find whether F. tularensis induces the antibody immune reaction in an early phase of infection (after 24 and 48 hours). The virulent strain of F. tularensis was used. We also intended to validate a feasibility of chosen methodology. Methods: We prepared a whole-cell bacteria lysate of F. tularensis holarctica FSC200. This lysate was separated by 2D electrophoresis with subsequent Western blotting. Proteins were marked by immunodetection using serum of BALB/c mice. Mice sera were collected 24 and 48 hours post challenge with F. tularensis. The serum of uninfected mice was utilized as control. Using comparative analysis, we tried to find proteins reacting only with the...