Vývoj umělého zubu založený na technice povrchvové plasmové resonance

• Main Author: Solanská, Kateřina
• Other Authors: Polášek, Miroslav
• Format: Dissertation
• Language: English
• Published: 2009
• Online Access: http://www.nusl.cz/ntk/nusl-279485

For development an artificial tooth hydroxyapatite nanoparticles solution was electrophoretically deposited on the gold chip. It was established that experimental conditions of solution preparation, electrically field have marked effect to thickness of the hydroxyapatite layer. By this way was prepared chip suitable for SPR measurements. In the firs step the bovine serum albumin (BSA) was chosen as the model protein shows absorption on the hydroxyapatite layer. Further the native saliva and artificial saliva were used for pellicle forming. Samples of saliva showed difference between binding behaviors on the hydroxyapatite layer. The application of chlorhexidingluconate pharmaceuticals on the pellicle shows increase in thickness but rinsing with native saliva disclosed that the components of chlorhexidingluconate pharmaceuticals can be easily removed from the surface.