Optimalizace podmínek pro kvalitativní a kvantitativní analýzu farnesolu a tyrosolu pomocí UPLC-MS/MS.

• Main Author: Greguš, Petr
• Other Authors: Šatínský, Dalibor
• Format: Dissertation
• Language: Czech
• Published: 2009
• Online Access: http://www.nusl.cz/ntk/nusl-279538

APLICATION OF UHPLC/MS/MS TECHNIQUE FOR QUALITATIVE AND QUANTITATIVE ANALYSIS OF FARNESOL AND TYROSOL, AS A PRODUCT OF METABOLISM OF CANDIDA ALBICANS Petr Gregus 1 , Lucie Novakova 1 1 Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Heyrovskeho 1203, 50005 Hradec Králové SUMMARY : Candida albicans is generally one of the most often isolated fungal pathogens in human body. It is a common cause of nosocomial infections, bloodstream infections, urinary infections and infections of digestive tract. One of the most infectious diseases is vaginal candidosis, which affects most of healthy population of women. Candida albicans can grow as hyphae, pseudohyphae, or budding yeast. The availability of this diversity - the variability of the internal morphology is of great benefit for the oraganism's lifestyle. Morphological change of yeast forms to pseudomycelium or mycelium (hyphae) characterizes the transition from colonial statute to invasive. In addition to the physical-chemical factors during these transformation procedures - farnesol and tyrosol participate as well as quorum sensing compounds. Farnesol which, if accumulated over a certain limit, it prevents the conversion of yeast to the mycelium. Tyrosol, which accelerates the conversion in to the form of the germ (germ tubes), namely the...