Využití monolitických kolon v kapilární kapalinové chromatografii
INTRODUCTION From the nineties of the twent5r's century [l], when appeared the first references about the new type ofthe stationary phases designated for HPLC called monoliths, these became to be the object of interest of several scientific groups [2-4]. Their uncontested advantage in compariso...
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Online Access: | http://www.nusl.cz/ntk/nusl-280367 |
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ndltd-nusl.cz-oai-invenio.nusl.cz-2803672018-06-21T04:40:41Z Využití monolitických kolon v kapilární kapalinové chromatografii Applications of monolithic columns in capillary liquid chromatography Holdšvendová, Petra Coufal, Pavel Pacáková, Věra Cvačka, Josef INTRODUCTION From the nineties of the twent5r's century [l], when appeared the first references about the new type ofthe stationary phases designated for HPLC called monoliths, these became to be the object of interest of several scientific groups [2-4]. Their uncontested advantage in comparison with classical columns is their high stability in pH range from 2 to 12 where there is no possibility to use the classical columns. other advantages are their easy and cheap preparation and the absence of frits on the ends of the separating medium because the monolith is covalently bound to the inner side of the capillary [5]. On the other hand, their disadvantage remains to be worse in reproducibility of the stationary phase preparation than in case of classical columns. This PhD Thesis is focused on monoliths based on organic polymers, mainly the methacrylate monoliths. For the first time, this ýpe of monoliths was shown by Peters et al. [6] and since then there have been made several monolithic phases based on different separating features 17_101. Methacrylate monoliths are convenient for separation of the low molecular substances L7l as well as the high molecular substances like the proteins, oligonucleotides or saccharides n0-121. 2009 info:eu-repo/semantics/doctoralThesis http://www.nusl.cz/ntk/nusl-280367 cze info:eu-repo/semantics/restrictedAccess |
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Czech |
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Doctoral Thesis |
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description |
INTRODUCTION From the nineties of the twent5r's century [l], when appeared the first references about the new type ofthe stationary phases designated for HPLC called monoliths, these became to be the object of interest of several scientific groups [2-4]. Their uncontested advantage in comparison with classical columns is their high stability in pH range from 2 to 12 where there is no possibility to use the classical columns. other advantages are their easy and cheap preparation and the absence of frits on the ends of the separating medium because the monolith is covalently bound to the inner side of the capillary [5]. On the other hand, their disadvantage remains to be worse in reproducibility of the stationary phase preparation than in case of classical columns. This PhD Thesis is focused on monoliths based on organic polymers, mainly the methacrylate monoliths. For the first time, this ýpe of monoliths was shown by Peters et al. [6] and since then there have been made several monolithic phases based on different separating features 17_101. Methacrylate monoliths are convenient for separation of the low molecular substances L7l as well as the high molecular substances like the proteins, oligonucleotides or saccharides n0-121. |
author2 |
Coufal, Pavel |
author_facet |
Coufal, Pavel Holdšvendová, Petra |
author |
Holdšvendová, Petra |
spellingShingle |
Holdšvendová, Petra Využití monolitických kolon v kapilární kapalinové chromatografii |
author_sort |
Holdšvendová, Petra |
title |
Využití monolitických kolon v kapilární kapalinové chromatografii |
title_short |
Využití monolitických kolon v kapilární kapalinové chromatografii |
title_full |
Využití monolitických kolon v kapilární kapalinové chromatografii |
title_fullStr |
Využití monolitických kolon v kapilární kapalinové chromatografii |
title_full_unstemmed |
Využití monolitických kolon v kapilární kapalinové chromatografii |
title_sort |
využití monolitických kolon v kapilární kapalinové chromatografii |
publishDate |
2009 |
url |
http://www.nusl.cz/ntk/nusl-280367 |
work_keys_str_mv |
AT holdsvendovapetra vyuzitimonolitickychkolonvkapilarnikapalinovechromatografii AT holdsvendovapetra applicationsofmonolithiccolumnsincapillaryliquidchromatography |
_version_ |
1718698099371671552 |