| Summary: | Kvítková L.: Evaluation of in vitro activity of potentially antifungal compounds by microdilution broth method - VIII. Over 250 new synthetized compounds divided into 16 chemical groups were tested by microdilution broth method for the establisment of in vitro antifungal activity. This method is based on the standard M27-A (Clinical Laboratory Standard Institute). RPMI 1640 medium buffered to pH 7.0 with MOPS was used and standardized inoculum was prepared from fresh fungal isolates grown on Sabouraud agar. All compounds tested were dissolved and diluted in DMSO. The MICs were determinated visually after 24 or 48 hours of incubation at 35C. The compounds were tested against eight fungal strains. The structure-activity relationships were studied and compared with the calculated log P values. Hydrophobicity of compounds (log P) was computed using the program ACD/LogP (Advanced Chemistry Development Inc., Ontario, Canada). The most active compounds were the derivatives of (E)-1-pyrazine-2- ylethane-1-one-thiosemicarbazone; the majority of the compounds showed moderate or high efficacy against all fungal strains tested. There were several compounds of the groups tested with a considerable antifungal effect, as well. The most susceptible fungal strain was Trichophyton mentagrophytes, on the contrary,...
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