Chirální separace acyklických nukleosid fosfonátů a stanovení asociačních konstant jejich komplexů s cyklodextriny kapilární elektroforézou

• Main Author: Mikysková, Hana
• Other Authors: Jelínek, Ivan
• Format: Dissertation
• Language: Czech
• Published: 2010
• Online Access: http://www.nusl.cz/ntk/nusl-284867

Capillary electrophoresis (CE) has been applied to the chiral separation of acyclic nucleoside phosphonates, which belong toof the most important already registered or potential antiviral drugs. For enantioseparation of acyclic nucleoside phosphonates, the following native and derivatized cyclodextrin were used as chiral selectors: ­cyclodextrin, ­cyclodextrin, ­cyclodextrin, heptakis(2,6­dimethyl)­ ­cyclodextrin, 2­hydroxypropyl­­cyclodextrin and quaternary ammonium ­cyclodextrine). The best resolution of (R,S)­enantiomers of acyclic nucleoside phosphonates was achieved in basic backgroung electrolyte composed of 35 mM sodium tetraborate; pH 10, with addition of 17.6 mM ­cyclodextrin or 10 mM quaternary ammonium ­cyclodextrin chiral selectors. Capillary electrophoresis has been employed for determination of association constants of complexes of acyclic nucleoside phosphonate enantiomers with above mentioned chiral selectors. The association constants were determined by nonlinear regression analysis from the experimentally measured dependence of effective electrophoretic mobilities of the analytes on concentration of the chiral selector in the background electrolyte. Prior to association constant calculation, the effective electrophoretic mobilities were corrected to reference temperature,...