Vliv hypoxie na embryonální vývoj myokardu a koronárního řečiště
Human, as all multicelullar organisms, is dependant on oxygen availability. Hypoxia is a stimulus which must the organism adapt from early development. In prenatal life fundaments of circulation originate separately and first than they interconnect in complete system of vessel tubes incorporating he...
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ndltd-nusl.cz-oai-invenio.nusl.cz-2856882017-09-02T04:15:35Z Vliv hypoxie na embryonální vývoj myokardu a koronárního řečiště Effect of hypoxia on embryonic development of the heart and coronary circulation Křížová, Petra Naňka, Ondřej Sedmera, David Human, as all multicelullar organisms, is dependant on oxygen availability. Hypoxia is a stimulus which must the organism adapt from early development. In prenatal life fundaments of circulation originate separately and first than they interconnect in complete system of vessel tubes incorporating heart. Haemodynamic - as one of the factors - soon takes part in vessels system development. Restricted blood flow results in vascular bed regression; on the contrary higher flow promotes its development in prenatal life as well as in adulthood. The aim of our study was to investigate the effect of hypoxia on coronary arterial tree formation and myocardial development and to explain embryonic lethality. Previous studies found ventricular dilatation and increased mortality with signs of heart failure. We used quail embryos (Coturnix coturnix japonica). Eggs were incubated under hypoxic conditions (16% O2). Controls were incubated in normal oxygen tension (21% O2). The effect of hypoxia was analyzed 5 - 9 day of embryo development. Coronary system was visualized by injecting Indian ink. Proliferation was measured using BrdU labelling. For histological analysis we used standard haematoxylin and eosin labelling. For further analysis we used immunohistochemical staining with antibodies against sarcomeric actin... 2007 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-285688 cze info:eu-repo/semantics/restrictedAccess |
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NDLTD |
language |
Czech |
format |
Dissertation |
sources |
NDLTD |
description |
Human, as all multicelullar organisms, is dependant on oxygen availability. Hypoxia is a stimulus which must the organism adapt from early development. In prenatal life fundaments of circulation originate separately and first than they interconnect in complete system of vessel tubes incorporating heart. Haemodynamic - as one of the factors - soon takes part in vessels system development. Restricted blood flow results in vascular bed regression; on the contrary higher flow promotes its development in prenatal life as well as in adulthood. The aim of our study was to investigate the effect of hypoxia on coronary arterial tree formation and myocardial development and to explain embryonic lethality. Previous studies found ventricular dilatation and increased mortality with signs of heart failure. We used quail embryos (Coturnix coturnix japonica). Eggs were incubated under hypoxic conditions (16% O2). Controls were incubated in normal oxygen tension (21% O2). The effect of hypoxia was analyzed 5 - 9 day of embryo development. Coronary system was visualized by injecting Indian ink. Proliferation was measured using BrdU labelling. For histological analysis we used standard haematoxylin and eosin labelling. For further analysis we used immunohistochemical staining with antibodies against sarcomeric actin... |
author2 |
Naňka, Ondřej |
author_facet |
Naňka, Ondřej Křížová, Petra |
author |
Křížová, Petra |
spellingShingle |
Křížová, Petra Vliv hypoxie na embryonální vývoj myokardu a koronárního řečiště |
author_sort |
Křížová, Petra |
title |
Vliv hypoxie na embryonální vývoj myokardu a koronárního řečiště |
title_short |
Vliv hypoxie na embryonální vývoj myokardu a koronárního řečiště |
title_full |
Vliv hypoxie na embryonální vývoj myokardu a koronárního řečiště |
title_fullStr |
Vliv hypoxie na embryonální vývoj myokardu a koronárního řečiště |
title_full_unstemmed |
Vliv hypoxie na embryonální vývoj myokardu a koronárního řečiště |
title_sort |
vliv hypoxie na embryonální vývoj myokardu a koronárního řečiště |
publishDate |
2007 |
url |
http://www.nusl.cz/ntk/nusl-285688 |
work_keys_str_mv |
AT krizovapetra vlivhypoxienaembryonalnivyvojmyokarduakoronarnihoreciste AT krizovapetra effectofhypoxiaonembryonicdevelopmentoftheheartandcoronarycirculation |
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1718524932741136384 |