Stanovení glukosaminu metodou kapilární zónové elektroforézy s bezkontaktní vodivostní detekcí
7.1.2. Determination of glucosamine using capillary zone electrophoresis with conductivity contactless detection A novel CE method with contactless conductivity detection suitable for the determination of glucosamine and K+ in pharmaceuticals was devised. Under the optimum conditions (aqueous 30 mM...
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ndltd-nusl.cz-oai-invenio.nusl.cz-2910272017-09-02T04:15:50Z Stanovení glukosaminu metodou kapilární zónové elektroforézy s bezkontaktní vodivostní detekcí Determination of glucosamine by capillary zone electrophoresis with contactless conductivity detection Los, Petr Polášek, Miroslav Pospíšilová, Marie 7.1.2. Determination of glucosamine using capillary zone electrophoresis with conductivity contactless detection A novel CE method with contactless conductivity detection suitable for the determination of glucosamine and K+ in pharmaceuticals was devised. Under the optimum conditions (aqueous 30 mM acetate buffer of pH 5.2 as the background electrolyte; voltage 30 kV; 25 řC) glucosamine (migrating as glucosaminium cation) was well separated from K+ that could occur in the dosage forms as excipient. The CE analysis was performed in fused-silica capillaries (50 μm i.d., 75 cm total length, 27 cm to detector) and the separation took < 3 min. The calibration graphs were linear for both glucosamine (100 - 300 μg/ml; rš = 0.997) and K+ (15 - 75 μg/ml; rš = 0.997) when using ethanolamine (100 μg/ml) as the internal standard. The LOD values (S/N = 3) were 9.3 μg/ml for glucosamine and 2.9 μg/ml for K+. The method was applied to the assay of glucosamine content in various dosage forms. Intermediate precision evaluated by determining the content of glucosamine in a single formulation on 3 consecutive days was characterized by RSD 2.35% (n=15). Acceptable accuracy of the CE method was confirmed by the added/found glucosamine recovery experiments (recoveries 94.6-103.3%) and by statistical comparison of the results... 2008 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-291027 cze info:eu-repo/semantics/restrictedAccess |
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Czech |
format |
Dissertation |
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NDLTD |
description |
7.1.2. Determination of glucosamine using capillary zone electrophoresis with conductivity contactless detection A novel CE method with contactless conductivity detection suitable for the determination of glucosamine and K+ in pharmaceuticals was devised. Under the optimum conditions (aqueous 30 mM acetate buffer of pH 5.2 as the background electrolyte; voltage 30 kV; 25 řC) glucosamine (migrating as glucosaminium cation) was well separated from K+ that could occur in the dosage forms as excipient. The CE analysis was performed in fused-silica capillaries (50 μm i.d., 75 cm total length, 27 cm to detector) and the separation took < 3 min. The calibration graphs were linear for both glucosamine (100 - 300 μg/ml; rš = 0.997) and K+ (15 - 75 μg/ml; rš = 0.997) when using ethanolamine (100 μg/ml) as the internal standard. The LOD values (S/N = 3) were 9.3 μg/ml for glucosamine and 2.9 μg/ml for K+. The method was applied to the assay of glucosamine content in various dosage forms. Intermediate precision evaluated by determining the content of glucosamine in a single formulation on 3 consecutive days was characterized by RSD 2.35% (n=15). Acceptable accuracy of the CE method was confirmed by the added/found glucosamine recovery experiments (recoveries 94.6-103.3%) and by statistical comparison of the results... |
author2 |
Polášek, Miroslav |
author_facet |
Polášek, Miroslav Los, Petr |
author |
Los, Petr |
spellingShingle |
Los, Petr Stanovení glukosaminu metodou kapilární zónové elektroforézy s bezkontaktní vodivostní detekcí |
author_sort |
Los, Petr |
title |
Stanovení glukosaminu metodou kapilární zónové elektroforézy s bezkontaktní vodivostní detekcí |
title_short |
Stanovení glukosaminu metodou kapilární zónové elektroforézy s bezkontaktní vodivostní detekcí |
title_full |
Stanovení glukosaminu metodou kapilární zónové elektroforézy s bezkontaktní vodivostní detekcí |
title_fullStr |
Stanovení glukosaminu metodou kapilární zónové elektroforézy s bezkontaktní vodivostní detekcí |
title_full_unstemmed |
Stanovení glukosaminu metodou kapilární zónové elektroforézy s bezkontaktní vodivostní detekcí |
title_sort |
stanovení glukosaminu metodou kapilární zónové elektroforézy s bezkontaktní vodivostní detekcí |
publishDate |
2008 |
url |
http://www.nusl.cz/ntk/nusl-291027 |
work_keys_str_mv |
AT lospetr stanoveniglukosaminumetodoukapilarnizonoveelektroforezysbezkontaktnivodivostnidetekci AT lospetr determinationofglucosaminebycapillaryzoneelectrophoresiswithcontactlessconductivitydetection |
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1718525092906926080 |