Summary: | Analytical evaluation of drugs by using chromatographic methods IV. Diploma Thesis Lenka Spáčilová Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Heyrovského 1203, Hradec Králové Rapid analytical methods are described to control quality of honeys, concerning residues of acaricides applied in hives to prevent the mite Varroa jacobsoni infestation. Tau - fluvalinate was analysed by the use of a SGX RP-18C column, 5 µm, a acetonitrile : water (40:60) mobile phase, flow rate of 1 ml/min and a UV detector set at 254 nm. An internal standard was used for quantitative determination. A calibration curve was constructed and verified, by means of which the concentration of tau - fluvalinate in honey can be determined. The elaborated method was validated by detection limit determination.
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