Analytické hodnocení účinných látek kapalinovou chromatografií

• Main Author: Janurová, Catherine
• Other Authors: Sochor, Jaroslav
• Format: Dissertation
• Language: Czech
• Published: 2008
• Online Access: http://www.nusl.cz/ntk/nusl-292523

Analytical determination of active compounds by liquid chromatography Diploma Thesis Catherine Janurová Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control, Heyrovského 1203, Hradec Králové This paper deals with the HPLC evaluation of dexamethasone in ointment base. Dexamethasone was analysed by the use of a C-18 column, a water: acetonitrile (40:60) mobile phase, flow rate of 0,6 ml/min and a UV detector set at 239 nm. An internal standard was used for quantitative determination. Acetonitrile was shown to be the best solvent for the isolation of dexamethasone from the ointment base. A calibration curve was constructed and verified, by means of which the concentration of dexamethasone in ointment was determined. The elaborated method was validated. Validation parameters: precision, accuracy, selectiveness, linearity, detection limit, quantitative limit and robustness were evaluated.