Hodnocení stability nových potenciálních léčiv v biologickém materiálu

• Main Author: Bechná, Lenka
• Other Authors: Kovaříková, Petra
• Format: Dissertation
• Language: Czech
• Published: 2008
• Online Access: http://www.nusl.cz/ntk/nusl-292568

High performance liquid chromatography (HPLC) is one of the most frequently used analytical techniques for the analysis of drugs. Pyridoxal isonicotinoyl hydrazone (PIH) is a parental compound of the new group of the selective iron chelators derived from aroylhydrazone, which are currently intensively investigated as promising drug candidates. Besides the ability to bind iron, PIH was shown to posses other pharmacological effects: cardioprotective, antioxidative and cytoprotective. This work deals with the investigation of the stability of water-soluble salt of PIH (PIH·2HCl) in three biological materials (rabbit plasma, BSA solution and ultrafiltrate) using HPLC. The half-lives of PIH in these media were calculated as well. The measurement was executed with the guard column Lichrocart - 4×4 mm I.D. (Purospher RP-18C, 5 µm) and the analytical column Lichrocart - 250×4 mm I.D. (Lichrospher 100, RP-18 C, 5 µm). The mobile phase was composed of methanol/acetonitrile (in the ratio 24:14) and phosphate buffer (NaH2PO4 0,01mol/l; pH 6,0 - adjusted by 10% solution NaOH) + EDTA (1,0 mmol/l), in the ratio 50:50. Flow rate was 1 ml/min. The detection was executed in the ultraviolet spectrum at the wavelength of 297 nm. The stability of the PIH was characterized by its half-lives in plasma - t 1/2 = 29 min., BSA...