Signalizace protein kinázy B a exprese cyklooxygenáz v časných fázích diabetické nefropatie
Introduction: Renal hypertrophy, extracellular matrix accumulation, aItered apoptosis as well as changes in regional hemodynamics have been implicated in the pathophysiology of nephropathy in diabetes mellitus (DM). On the molecular level the detailed mechanisms for development of diabetic nephropat...
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2008
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ndltd-nusl.cz-oai-invenio.nusl.cz-2949602021-02-26T05:19:27Z Signalizace protein kinázy B a exprese cyklooxygenáz v časných fázích diabetické nefropatie Signalling of protein kinase B and expression of cyclooxygenase in early phases of diabetic nephropathy Ždychová, Jana Komers, Radko Haluzík, Martin Matoušovic, Karel Škrha, Jan Introduction: Renal hypertrophy, extracellular matrix accumulation, aItered apoptosis as well as changes in regional hemodynamics have been implicated in the pathophysiology of nephropathy in diabetes mellitus (DM). On the molecular level the detailed mechanisms for development of diabetic nephropathy (DN) have becn intensively studied. Insulin induces a variety of biologicaI effects in a number of cell types via phosphatidylinositol-3 kinase (PI3K)/Akt kinase signaling pathway. Considering multiple function of Akt that incIude potentiaIly hannful pro-growth effects mediated by mTOR and cyclooxygenas-2 (COX-2), as well as protective effects mediated by endotheliaI nitric oxide synthase (eNOS), it is possible that aIterations in activities of Akt may play role in the pathophysiology ofDN. Renal corticaI activity and expression of Akt, its down-strearn effectors mTOR, eNOS, and "C<JIX-:Z. as well as PTEN, an endogenous Akt inhibitor, were investigated in streptozotocin (STZ): diabetic rats as a model of Type 1 DM with different levels of glycenůc control, and in Zucker ~d.iabetic fatty rats, a model ofDM2, and in nondiabetic rats as controls. Methods: Akt activity was measured by kinase assay. Protein expressions were measured by .immunoblotting and immunohistochemistry in renal cortex of 4- and 12- week old... 2008 info:eu-repo/semantics/doctoralThesis http://www.nusl.cz/ntk/nusl-294960 cze info:eu-repo/semantics/restrictedAccess |
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Czech |
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Doctoral Thesis |
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description |
Introduction: Renal hypertrophy, extracellular matrix accumulation, aItered apoptosis as well as changes in regional hemodynamics have been implicated in the pathophysiology of nephropathy in diabetes mellitus (DM). On the molecular level the detailed mechanisms for development of diabetic nephropathy (DN) have becn intensively studied. Insulin induces a variety of biologicaI effects in a number of cell types via phosphatidylinositol-3 kinase (PI3K)/Akt kinase signaling pathway. Considering multiple function of Akt that incIude potentiaIly hannful pro-growth effects mediated by mTOR and cyclooxygenas-2 (COX-2), as well as protective effects mediated by endotheliaI nitric oxide synthase (eNOS), it is possible that aIterations in activities of Akt may play role in the pathophysiology ofDN. Renal corticaI activity and expression of Akt, its down-strearn effectors mTOR, eNOS, and "C<JIX-:Z. as well as PTEN, an endogenous Akt inhibitor, were investigated in streptozotocin (STZ): diabetic rats as a model of Type 1 DM with different levels of glycenůc control, and in Zucker ~d.iabetic fatty rats, a model ofDM2, and in nondiabetic rats as controls. Methods: Akt activity was measured by kinase assay. Protein expressions were measured by .immunoblotting and immunohistochemistry in renal cortex of 4- and 12- week old... |
author2 |
Komers, Radko |
author_facet |
Komers, Radko Ždychová, Jana |
author |
Ždychová, Jana |
spellingShingle |
Ždychová, Jana Signalizace protein kinázy B a exprese cyklooxygenáz v časných fázích diabetické nefropatie |
author_sort |
Ždychová, Jana |
title |
Signalizace protein kinázy B a exprese cyklooxygenáz v časných fázích diabetické nefropatie |
title_short |
Signalizace protein kinázy B a exprese cyklooxygenáz v časných fázích diabetické nefropatie |
title_full |
Signalizace protein kinázy B a exprese cyklooxygenáz v časných fázích diabetické nefropatie |
title_fullStr |
Signalizace protein kinázy B a exprese cyklooxygenáz v časných fázích diabetické nefropatie |
title_full_unstemmed |
Signalizace protein kinázy B a exprese cyklooxygenáz v časných fázích diabetické nefropatie |
title_sort |
signalizace protein kinázy b a exprese cyklooxygenáz v časných fázích diabetické nefropatie |
publishDate |
2008 |
url |
http://www.nusl.cz/ntk/nusl-294960 |
work_keys_str_mv |
AT zdychovajana signalizaceproteinkinazybaexpresecyklooxygenazvcasnychfazichdiabetickenefropatie AT zdychovajana signallingofproteinkinasebandexpressionofcyclooxygenaseinearlyphasesofdiabeticnephropathy |
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1719379298939305984 |