HPLC hodnocení vybraných léčiv IV.

• Main Author: Soukupová, Petra
• Other Authors: Mokrý, Milan
• Format: Dissertation
• Language: Czech
• Published: 2011
• Online Access: http://www.nusl.cz/ntk/nusl-296767

HPLC evaluation of some drugs IV. THESIS Petra Soukupová Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Analysis This thesis is about looking for the optimal conditions for simultaneous HPLC analysis in combined tablets of perindopril and indapamid with subsequent determination of their content. As the most suitable stacionary phase chromatographic column LiChro CART 250-4 LiChrospher 100, RP18, 5 µm was chosen, and the mobile phase formed by mixture of acetonitrile - ammonium phosphate solution (0,05 mol/l) in the ratio 40:60 with addition of triethylamine (0,1 ml/100 ml) acidified with phosporic acid at pH 3 at a flow rate of 1,4 ml/min and temperature 50o C. Propylparaben was used as the most suitable internal standard. The detection was performed at 215 nm using an UV detector. At the determination of content of medicaments in tablets was found that used compounds were eluted in following order: perindopril, indapamid and propylparaben.