Optimalizace purifikace lidské membránově vázané karbonylreduktasy
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Rudolf Andrýs Supervisor: prof. Ing. Vladimír Wsól, Ph.D Title of diploma thesis: Optimalization of purification of a human membrane-bound carbonyl reductase Carbonyl reductases are enzym...
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Online Access: | http://www.nusl.cz/ntk/nusl-297602 |
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ndltd-nusl.cz-oai-invenio.nusl.cz-2976022017-06-27T04:42:32Z Optimalizace purifikace lidské membránově vázané karbonylreduktasy Optimization of purification of human membrane-bound carbonyl reductase. Andrýs, Rudolf Wsól, Vladimír Bílková, Zuzana Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Rudolf Andrýs Supervisor: prof. Ing. Vladimír Wsól, Ph.D Title of diploma thesis: Optimalization of purification of a human membrane-bound carbonyl reductase Carbonyl reductases are enzymes participating in metabolic pathways of various eobiotics and xenobiotics. Of all known enzymes metabolizing xenobiotics only 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) is found in the microsomal membrane. It also contributes to metabolism of prochiral anticancer drug oracin, which main metabolic pathway is a carbonyl reduction on the position 11 leading to two enantiomers of (+) an (-) 11-dihydrooracin (DHO). Based on the discrepancy between microsomes and 11β-HSD1 stereospecifity of oracin reduction exist a hypothesis of participation an unknown microsomal enzyme in oracin metabolism. The aim of this study is to purify a new microsomal carbonyl reducing enzyme contributing in the biotransformation of oracin. Human liver microsomes were solubilised and desalted. The prepared sample was used for the first purification step on Q-Sepharose. Captured flow through fraction Q2 was loaded on Phenyl-sepharose. Captured suitable fraction P11 was used for third purification step by gel filtration. All... 2011 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-297602 cze info:eu-repo/semantics/restrictedAccess |
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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Rudolf Andrýs Supervisor: prof. Ing. Vladimír Wsól, Ph.D Title of diploma thesis: Optimalization of purification of a human membrane-bound carbonyl reductase Carbonyl reductases are enzymes participating in metabolic pathways of various eobiotics and xenobiotics. Of all known enzymes metabolizing xenobiotics only 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) is found in the microsomal membrane. It also contributes to metabolism of prochiral anticancer drug oracin, which main metabolic pathway is a carbonyl reduction on the position 11 leading to two enantiomers of (+) an (-) 11-dihydrooracin (DHO). Based on the discrepancy between microsomes and 11β-HSD1 stereospecifity of oracin reduction exist a hypothesis of participation an unknown microsomal enzyme in oracin metabolism. The aim of this study is to purify a new microsomal carbonyl reducing enzyme contributing in the biotransformation of oracin. Human liver microsomes were solubilised and desalted. The prepared sample was used for the first purification step on Q-Sepharose. Captured flow through fraction Q2 was loaded on Phenyl-sepharose. Captured suitable fraction P11 was used for third purification step by gel filtration. All... |
author2 |
Wsól, Vladimír |
author_facet |
Wsól, Vladimír Andrýs, Rudolf |
author |
Andrýs, Rudolf |
spellingShingle |
Andrýs, Rudolf Optimalizace purifikace lidské membránově vázané karbonylreduktasy |
author_sort |
Andrýs, Rudolf |
title |
Optimalizace purifikace lidské membránově vázané karbonylreduktasy |
title_short |
Optimalizace purifikace lidské membránově vázané karbonylreduktasy |
title_full |
Optimalizace purifikace lidské membránově vázané karbonylreduktasy |
title_fullStr |
Optimalizace purifikace lidské membránově vázané karbonylreduktasy |
title_full_unstemmed |
Optimalizace purifikace lidské membránově vázané karbonylreduktasy |
title_sort |
optimalizace purifikace lidské membránově vázané karbonylreduktasy |
publishDate |
2011 |
url |
http://www.nusl.cz/ntk/nusl-297602 |
work_keys_str_mv |
AT andrysrudolf optimalizacepurifikacelidskemembranovevazanekarbonylreduktasy AT andrysrudolf optimizationofpurificationofhumanmembraneboundcarbonylreductase |
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1718471146204037120 |