Analýza eskulinu, eskuletinu a fenylefrinu v očních kapkách pomocí micelární elektrokinetické chromatografie
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Lucie Pincová Supervisor: Doc. RNDr. Marie Pospíšilová, CSc. Title of Diploma Thesis: Analysis of aesculin, aesculetin and phenylephrine in eye drops by micellar electrokinetic chromatog...
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Online Access: | http://www.nusl.cz/ntk/nusl-297675 |
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ndltd-nusl.cz-oai-invenio.nusl.cz-2976752017-06-27T04:42:32Z Analýza eskulinu, eskuletinu a fenylefrinu v očních kapkách pomocí micelární elektrokinetické chromatografie Analysis of aesculin, aesculetin and phenylephrine in eye drops by micellar electrokinetic chromatography Pincová, Lucie Pospíšilová, Marie Polášek, Miroslav Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Lucie Pincová Supervisor: Doc. RNDr. Marie Pospíšilová, CSc. Title of Diploma Thesis: Analysis of aesculin, aesculetin and phenylephrine in eye drops by micellar electrokinetic chromatography A new method of micellar electrokinetic chromatography with UV detection for the simultaneous determination of aesculin, aesculetin and phenylephrine was devised and validated. The separation was optimized by examining a number of experimental conditions, such as type, concentration and pH of the background electrolyte, the addition of a surface-active agent (sodium dodecyl sulfate, SDS) and methanol and effect of applied voltage and operating temperature. The separation was carried out in a fused-silica capillary (internal diameter 50 µm, total length 64.5 cm and effective length 8.5 cm) with UV detection at 210 nm, using hydrodynamic injection under the pressure of −50 mbar for 6 seconds. Optimal conditions for the separation and determination of the drugs were: background electrolyte 20mM boric acid of pH 8.6 (adjusted with 100mM NaOH) containing 60 mM SDS and 5 % (V/V) of methanol. Benzoic acid (0.1 mg/ml) was used as internal standard. The capillary temperature was maintained at 25 řC, the... 2011 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-297675 cze info:eu-repo/semantics/restrictedAccess |
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Dissertation |
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Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Lucie Pincová Supervisor: Doc. RNDr. Marie Pospíšilová, CSc. Title of Diploma Thesis: Analysis of aesculin, aesculetin and phenylephrine in eye drops by micellar electrokinetic chromatography A new method of micellar electrokinetic chromatography with UV detection for the simultaneous determination of aesculin, aesculetin and phenylephrine was devised and validated. The separation was optimized by examining a number of experimental conditions, such as type, concentration and pH of the background electrolyte, the addition of a surface-active agent (sodium dodecyl sulfate, SDS) and methanol and effect of applied voltage and operating temperature. The separation was carried out in a fused-silica capillary (internal diameter 50 µm, total length 64.5 cm and effective length 8.5 cm) with UV detection at 210 nm, using hydrodynamic injection under the pressure of −50 mbar for 6 seconds. Optimal conditions for the separation and determination of the drugs were: background electrolyte 20mM boric acid of pH 8.6 (adjusted with 100mM NaOH) containing 60 mM SDS and 5 % (V/V) of methanol. Benzoic acid (0.1 mg/ml) was used as internal standard. The capillary temperature was maintained at 25 řC, the... |
author2 |
Pospíšilová, Marie |
author_facet |
Pospíšilová, Marie Pincová, Lucie |
author |
Pincová, Lucie |
spellingShingle |
Pincová, Lucie Analýza eskulinu, eskuletinu a fenylefrinu v očních kapkách pomocí micelární elektrokinetické chromatografie |
author_sort |
Pincová, Lucie |
title |
Analýza eskulinu, eskuletinu a fenylefrinu v očních kapkách pomocí micelární elektrokinetické chromatografie |
title_short |
Analýza eskulinu, eskuletinu a fenylefrinu v očních kapkách pomocí micelární elektrokinetické chromatografie |
title_full |
Analýza eskulinu, eskuletinu a fenylefrinu v očních kapkách pomocí micelární elektrokinetické chromatografie |
title_fullStr |
Analýza eskulinu, eskuletinu a fenylefrinu v očních kapkách pomocí micelární elektrokinetické chromatografie |
title_full_unstemmed |
Analýza eskulinu, eskuletinu a fenylefrinu v očních kapkách pomocí micelární elektrokinetické chromatografie |
title_sort |
analýza eskulinu, eskuletinu a fenylefrinu v očních kapkách pomocí micelární elektrokinetické chromatografie |
publishDate |
2011 |
url |
http://www.nusl.cz/ntk/nusl-297675 |
work_keys_str_mv |
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