Nové matrice pro MALDI-MS analýzu lipidů
In this work focused on research of new matrices for MALDI-MS analysis of lipids several organic salts were tested as potential matrices for hydrocarbons and wax esters. It was shown previously that the effective ionization of lipids occurs in conjuction with a lithium cation to form adducts [M + Li...
Main Author: | |
---|---|
Other Authors: | |
Format: | Dissertation |
Language: | Czech |
Published: |
2011
|
Online Access: | http://www.nusl.cz/ntk/nusl-298002 |
id |
ndltd-nusl.cz-oai-invenio.nusl.cz-298002 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-nusl.cz-oai-invenio.nusl.cz-2980022021-03-29T05:12:13Z Nové matrice pro MALDI-MS analýzu lipidů New matrices for MALDI-MS analysis of lipids Macháčková, Petra Cvačka, Josef Volný, Michael In this work focused on research of new matrices for MALDI-MS analysis of lipids several organic salts were tested as potential matrices for hydrocarbons and wax esters. It was shown previously that the effective ionization of lipids occurs in conjuction with a lithium cation to form adducts [M + Li] + . Therefore, lithium salts of aromatic acids were synthesized. Matrices were evaluated according to their MALDI properties and compared with lithium 2,5-dihydroxybenzoate, which is proven matrix for MALDI analysis of lipids. It was found that effectiveness of matrix depends on presence of hydroxyl group in matrix molecule. The best results were achieved with lithium vannilate, even in comparison with lithium 2,5-dihydroxybenzoate. 2011 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-298002 cze info:eu-repo/semantics/restrictedAccess |
collection |
NDLTD |
language |
Czech |
format |
Dissertation |
sources |
NDLTD |
description |
In this work focused on research of new matrices for MALDI-MS analysis of lipids several organic salts were tested as potential matrices for hydrocarbons and wax esters. It was shown previously that the effective ionization of lipids occurs in conjuction with a lithium cation to form adducts [M + Li] + . Therefore, lithium salts of aromatic acids were synthesized. Matrices were evaluated according to their MALDI properties and compared with lithium 2,5-dihydroxybenzoate, which is proven matrix for MALDI analysis of lipids. It was found that effectiveness of matrix depends on presence of hydroxyl group in matrix molecule. The best results were achieved with lithium vannilate, even in comparison with lithium 2,5-dihydroxybenzoate. |
author2 |
Cvačka, Josef |
author_facet |
Cvačka, Josef Macháčková, Petra |
author |
Macháčková, Petra |
spellingShingle |
Macháčková, Petra Nové matrice pro MALDI-MS analýzu lipidů |
author_sort |
Macháčková, Petra |
title |
Nové matrice pro MALDI-MS analýzu lipidů |
title_short |
Nové matrice pro MALDI-MS analýzu lipidů |
title_full |
Nové matrice pro MALDI-MS analýzu lipidů |
title_fullStr |
Nové matrice pro MALDI-MS analýzu lipidů |
title_full_unstemmed |
Nové matrice pro MALDI-MS analýzu lipidů |
title_sort |
nové matrice pro maldi-ms analýzu lipidů |
publishDate |
2011 |
url |
http://www.nusl.cz/ntk/nusl-298002 |
work_keys_str_mv |
AT machackovapetra novematricepromaldimsanalyzulipidu AT machackovapetra newmatricesformaldimsanalysisoflipids |
_version_ |
1719389197738967040 |