Stanovení laktulosy pomocí kapilární elektroforézy

• Main Author: Holcová, Kristýna
• Other Authors: Doležal, Martin
• Format: Dissertation
• Language: German
• Published: 2011
• Online Access: http://www.nusl.cz/ntk/nusl-298056

Determination of Lactulose by capillary zone electrophoresis Kristýna Holcová A lot of sugar analysis methods exist. Capillary Electophoresis (CE), High Performance Liquid Chromatography (HPLC), Gas Chromatogramy (GC), Thin-Layer Chromatography (TLC) etc. HPLC is the method which is most used, however other methods have also some advantage. One of the already described capillary electrophoresis methods is the carbohydrate separation in alkaline medium after chelatation with copper(II) ions with direct UV detection without need of previous derivatization. It has been tested for simple sugars, disaccharides, some amine-acids and inorganic cations. I applied capillary electrophoresis method mentioned above on questions of setting up osmotic laxancium lactulose. Lactulose is disaccharide from the chemistry point of view. It appears in molasses form in commercial medicaments. The method has been suited to the apparature used during the research and tested substance. It has been proved the specifity and accuracy of the method and it has been set up correlative factor (0,9994). Quality and quantity analysis has been applied on chosen samples.