Aplikace kapalinové chromatografie za ultravysokého tlaku a hmotnostní spektrometrie v analýze polyfenolických látek

• Main Author: Spáčil, Zdeněk
• Other Authors: Solich, Petr
• Format: Doctoral Thesis
• Language: Czech
• Published: 2010
• Online Access: http://www.nusl.cz/ntk/nusl-299745

DOCTORAL THESIS ABSTRACT 7 ABSTRACT Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Zdeněk Spáčil, M.Sc. Supervisor: prof. RNDr. Petr Solich, CSc. Title of Doctoral Thesis: Application of Ultra-High Pressure Liquid Chromatography with Mass Spectrometry Detection in Analysis of Polyphenols The major focus of presented doctoral thesis is development of advanced separation methods utilizing liquid chromatography (LC) and mass spectrometry (MS) for the analysis of polyphenols. The general aspects of techniques are discussed in theoretical part and the actual practical impact is documented by the results published in four original articles appended in the supplement I-IV. LC was used in each published study, while combined with MS in the last two works (supplement III-IV). Thus, the semi-quantitative data acquired by emerging laser desorption/ionization (LDI) MS technique were confirmed by UHPLC-MS. The broad availability, versatile configuration, convenient handling and well understood principles make LC the "method of choice" for variety of scientific applications to analyze diverse analytes. LC-MS coupling is convenient and robust offering two-staged separation: i) chromatographic separation and pre-concentration into narrow bands using LC and...