Stanovení nízkých koncentrací kationogenních povrchově aktivních látek ve vodách technikou průtokové injekční analýzy se spektrofluorimetrickou detekcí
This thesis was focused on a development of sensitive determination of cationactive tenzides by using the flow injection analyses with spectrofluorimetric detection. The determination was based on an interaction of cationactive tenzides with sodium salt of 4,5,6,7-tetrachloro-2',4',5'...
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2012
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Online Access: | http://www.nusl.cz/ntk/nusl-305427 |
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ndltd-nusl.cz-oai-invenio.nusl.cz-3054272017-06-28T04:15:54Z Stanovení nízkých koncentrací kationogenních povrchově aktivních látek ve vodách technikou průtokové injekční analýzy se spektrofluorimetrickou detekcí Determination of Low Concentrations of Cationactive Tenzides in Water Samples using Flow Injection Analysis Technique with Spectrofluorimetric Detection Havelka, Michal Rychlovský, Petr Červený, Václav This thesis was focused on a development of sensitive determination of cationactive tenzides by using the flow injection analyses with spectrofluorimetric detection. The determination was based on an interaction of cationactive tenzides with sodium salt of 4,5,6,7-tetrachloro-2',4',5',7'- tetraiodofluorescein dye (Bengal red) that generates a soluble associate in water environs. The sodium salt of 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein is a fluorescence-active compound. A decrease of fluorescence intensity is detected as a result of the formation of fluorescence-inactive associate. A development of the determination proceeded in two steps. At first a static method of the determination was developed. Cetyltrimethylammonium bromide (CTAB) and N-(α-karbethoxypentadecyl)trimethyl ammonium bromide (Septonex) were used as a standard for cationactive tenzides. An optimal theoretical concentration of Bengal red dye (5.10-5 mol/l) was found, an optimum rate of pH for the fluorescence of Bengal red dye (pH = 9) was found, and a suitable kind of buffer for the environs of the interactions (25 mM phosphate buffer) was found. A linear dynamic range for the determination (Septonex) was in the interval: the limit of quantification - 7.10-5 mol/l. The limit of quantification was 3,72.10-6 mol/l. Next... 2012 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-305427 cze info:eu-repo/semantics/restrictedAccess |
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NDLTD |
language |
Czech |
format |
Dissertation |
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NDLTD |
description |
This thesis was focused on a development of sensitive determination of cationactive tenzides by using the flow injection analyses with spectrofluorimetric detection. The determination was based on an interaction of cationactive tenzides with sodium salt of 4,5,6,7-tetrachloro-2',4',5',7'- tetraiodofluorescein dye (Bengal red) that generates a soluble associate in water environs. The sodium salt of 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein is a fluorescence-active compound. A decrease of fluorescence intensity is detected as a result of the formation of fluorescence-inactive associate. A development of the determination proceeded in two steps. At first a static method of the determination was developed. Cetyltrimethylammonium bromide (CTAB) and N-(α-karbethoxypentadecyl)trimethyl ammonium bromide (Septonex) were used as a standard for cationactive tenzides. An optimal theoretical concentration of Bengal red dye (5.10-5 mol/l) was found, an optimum rate of pH for the fluorescence of Bengal red dye (pH = 9) was found, and a suitable kind of buffer for the environs of the interactions (25 mM phosphate buffer) was found. A linear dynamic range for the determination (Septonex) was in the interval: the limit of quantification - 7.10-5 mol/l. The limit of quantification was 3,72.10-6 mol/l. Next... |
author2 |
Rychlovský, Petr |
author_facet |
Rychlovský, Petr Havelka, Michal |
author |
Havelka, Michal |
spellingShingle |
Havelka, Michal Stanovení nízkých koncentrací kationogenních povrchově aktivních látek ve vodách technikou průtokové injekční analýzy se spektrofluorimetrickou detekcí |
author_sort |
Havelka, Michal |
title |
Stanovení nízkých koncentrací kationogenních povrchově aktivních látek ve vodách technikou průtokové injekční analýzy se spektrofluorimetrickou detekcí |
title_short |
Stanovení nízkých koncentrací kationogenních povrchově aktivních látek ve vodách technikou průtokové injekční analýzy se spektrofluorimetrickou detekcí |
title_full |
Stanovení nízkých koncentrací kationogenních povrchově aktivních látek ve vodách technikou průtokové injekční analýzy se spektrofluorimetrickou detekcí |
title_fullStr |
Stanovení nízkých koncentrací kationogenních povrchově aktivních látek ve vodách technikou průtokové injekční analýzy se spektrofluorimetrickou detekcí |
title_full_unstemmed |
Stanovení nízkých koncentrací kationogenních povrchově aktivních látek ve vodách technikou průtokové injekční analýzy se spektrofluorimetrickou detekcí |
title_sort |
stanovení nízkých koncentrací kationogenních povrchově aktivních látek ve vodách technikou průtokové injekční analýzy se spektrofluorimetrickou detekcí |
publishDate |
2012 |
url |
http://www.nusl.cz/ntk/nusl-305427 |
work_keys_str_mv |
AT havelkamichal stanoveninizkychkoncentracikationogennichpovrchoveaktivnichlatekvevodachtechnikouprutokoveinjekcnianalyzysespektrofluorimetrickoudetekci AT havelkamichal determinationoflowconcentrationsofcationactivetenzidesinwatersamplesusingflowinjectionanalysistechniquewithspectrofluorimetricdetection |
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1718473104787767296 |