Hodnocení cytotoxických účinků vybraných akcelerantů transdermální penetrace u keratinocytů

• Main Author: Wojnarová, Lea
• Other Authors: Šimůnek, Tomáš
• Format: Dissertation
• Language: Czech
• Published: 2012
• Online Access: http://www.nusl.cz/ntk/nusl-306717

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciencis Candidate: Lea Wojnarová Supervisor: Doc. PharmDr. Tomáš Šimůnek, Ph.D. Title of diploma thesis: Assessment of the cytotoxic effects of selected transdermal permeation enhancers in keratinocytes Only a few drugs are able to permeate through the skin and reach therapeutic concentration in the systemic circulation. One method to enable transdermal administration can be the use of accelerants transdermal penetration. These substances affect different mechanisms and reversibly reduce the barrier properties of the skin. Substances which are used in clinical practice must fulfill strict criteria which include: efficiency and compatibility of the drug and accelerants; it must be non-toxic; non- irritating and without own pharmacological effect. In our research we studied selected transdermal penetration enhancers, namely Azone, dodecanol, DDAIP, DDAK, Transkarbam 12 and new derivatives of amino acids proline - LP12/2 and DP12/2 enantiomers and sarcosine - 12Sar2. The aim of our study was to determine the cytotoxicity effect of accelerants and assess whether the apoptosis was activated. These effects were studied using the keratinocyte cell line HaCaT. To determine the cytotoxic properties of accelerants,...