Vliv Spirulina platensis na endoteliální expresi eNOS u myší
Beata Rusnoková Spirulina platensis effects on endothelial expression of in mice Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Background: We observed the endothelial expression of eNOS in aorta of ApoE- deficient mice after 8 weeks long administration o...
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| Format: | Dissertation |
| Language: | Czech |
| Published: |
2012
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| Online Access: | http://www.nusl.cz/ntk/nusl-307129 |
| Summary: | Beata Rusnoková Spirulina platensis effects on endothelial expression of in mice Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Background: We observed the endothelial expression of eNOS in aorta of ApoE- deficient mice after 8 weeks long administration of Spirulina platensis. The expression was assessed by using immunohistochemical methods and quantified by means of stereological methods. Methods: In the experiment, we used C57BL/6J male mice with deficiency of apolipoprotein E (apoE-/-) that were divided into two groups. The control group was fed by atherogenic diet with 1 % cholesterol for 8 weeks. Mice from Spirulina platensis group was fed by the same atherogenic diet with the addition of 40 mg Spirulina platensis. We performed a biochemical analysis of blood. The immunohistochemical analysis was performed in a 1 cm aortic sinus and the aortic arch. A series of cross sections with a thickness of 7 µm were to evaluate. The detection of expression of eNOS was performed by Envision methods by means of DAB. Results: The biochemical analysis did not show significant changes in total cholesterol levels in the control mice compared with the group administrated by Spirulina. The marked expression was observed in all mice in the experiment. There were no... |
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