| Summary: | Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Candidate: Barbora Kadaňková Consultant: RNDr. Milan Mokrý, CSc. Title of Thesis: HPLC hodnocení vybraných léčiv VI. This thesis focuses on assay optimization for simultaneous quantitation of valsartan and hydrochlorothiazide in combined tablets. First, chromatographic conditions were tested and optimized settings were then used for tablet content determination. Good chromatographic separation was achieved using an ET Nucleosil (125x4.6 mm, 120-5, C8) column and a mobile phase consisting of acetonitrile : ammonium acetate (0.001 mol/l) in the ratio of 25:75 (v/v) with addition of sodium hexanesulfonate (0.002 mol/l). The solution was acidified with acetic acid to pH 3.50 and then adjusted to pH 7.00 using disodium hydrogen phosphate. The mobile phase was pumped at a flow rate of 1.0 ml/min and UV detector was set at 254 nm. Methylparaben was used as internal standard. Individual compounds eluted in the following order: 1) hydrochlorothiazide, 2) valsartan and 3) methylparaben. The linearity range for valsartan and hydrochlorothiazide quantitation was 0.08-0.24 mg/ml and 0.012-0.036 mg/ml, respectively.
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