Klonování, exprese a purifikace lidské 17beta-HSD1
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Lenka Popovská Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Title of diploma thesis: Cloning, expression and purification of human 17β-HSD1 17β-hydroxysteroid dehydrogenase type 1 (17β...
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ndltd-nusl.cz-oai-invenio.nusl.cz-3101872017-06-28T04:16:23Z Klonování, exprese a purifikace lidské 17beta-HSD1 Cloning, expression and purification of human 17beta-HSD1 Popovská, Lenka Wsól, Vladimír Novotná, Eva Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Lenka Popovská Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Title of diploma thesis: Cloning, expression and purification of human 17β-HSD1 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1; SDR28C1; EC 1.1.1.62) belongs to the SDR superfamily and catalyzes the NAD(P)(H) dependent oxidoreduction of hydroxyl/keto groups at position C17 of androgens and estrogens and in this manner regulate intracellular availability of steroid hormone ligands to their nuclear receptors. This pathway constitutes a pre-receptor control mechanism. Estradiol is the most potent female sex steroid and the only one responsible for estrogen action in women. The ovary is the primary source of estradiol present in the circulation of premenopausal women, but circulating estrone and androgens originating from the adrenal gland are also converted to estradiol in peripheral tissues such as adipose tissue. After menopause, estrogen biosynthesis in peripheral tissues has a major role in estrogen action. For production of recombinant protein 17β-HSD1 bacterial strain E. coli DH10B with inserted vector pOTB7, which contained coding sequence of the respective enzyme, was used. The sequence of the enzyme was isolated from... 2012 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-310187 cze info:eu-repo/semantics/restrictedAccess |
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Dissertation |
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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Lenka Popovská Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Title of diploma thesis: Cloning, expression and purification of human 17β-HSD1 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1; SDR28C1; EC 1.1.1.62) belongs to the SDR superfamily and catalyzes the NAD(P)(H) dependent oxidoreduction of hydroxyl/keto groups at position C17 of androgens and estrogens and in this manner regulate intracellular availability of steroid hormone ligands to their nuclear receptors. This pathway constitutes a pre-receptor control mechanism. Estradiol is the most potent female sex steroid and the only one responsible for estrogen action in women. The ovary is the primary source of estradiol present in the circulation of premenopausal women, but circulating estrone and androgens originating from the adrenal gland are also converted to estradiol in peripheral tissues such as adipose tissue. After menopause, estrogen biosynthesis in peripheral tissues has a major role in estrogen action. For production of recombinant protein 17β-HSD1 bacterial strain E. coli DH10B with inserted vector pOTB7, which contained coding sequence of the respective enzyme, was used. The sequence of the enzyme was isolated from... |
author2 |
Wsól, Vladimír |
author_facet |
Wsól, Vladimír Popovská, Lenka |
author |
Popovská, Lenka |
spellingShingle |
Popovská, Lenka Klonování, exprese a purifikace lidské 17beta-HSD1 |
author_sort |
Popovská, Lenka |
title |
Klonování, exprese a purifikace lidské 17beta-HSD1 |
title_short |
Klonování, exprese a purifikace lidské 17beta-HSD1 |
title_full |
Klonování, exprese a purifikace lidské 17beta-HSD1 |
title_fullStr |
Klonování, exprese a purifikace lidské 17beta-HSD1 |
title_full_unstemmed |
Klonování, exprese a purifikace lidské 17beta-HSD1 |
title_sort |
klonování, exprese a purifikace lidské 17beta-hsd1 |
publishDate |
2012 |
url |
http://www.nusl.cz/ntk/nusl-310187 |
work_keys_str_mv |
AT popovskalenka klonovaniexpreseapurifikacelidske17betahsd1 AT popovskalenka cloningexpressionandpurificationofhuman17betahsd1 |
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1718473658687553536 |