| Summary: | Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Michala Vařejčková Supervisor: PhDr. Zdeňka Kudláčková, Ph.D. Consultant: prof. MUDr. Jaroslav Mokrý, Ph.D. Title of thesis: Detection of nestin in tissues Aim of the work: The aim of this study was to use immunohistochemical methods to show intermediate filament nestin occurs not only in the capillaries but also in large blood vessels - arteries and veins - which has not been published so far. We also investigated coexpression of nestin with a proliferating marker, PCNA. Methods: All studies were performed using embryos 11 and 16 days old from Wistar rats (VELAZ, Prague, Czech Republic). Expression of nestin and PCNA was detected by various types of indirect immunohistochemical method. To amplify a specific signal, we used EnVision system or biotinylated tyramide. Main results: Endothelium of blood vessels expressed nestin with a varying intensity. In some blood vessels expression of nestin was missing. In a developing tissue, nestin is gradually being replaced by increased expression of another type of intermediate filament. To identify tissue antigens that are expressed in a weak intensity, it is better to use signal amplification with tyramide. Conclusion: The results of this study...
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